| Shu Huang,Ganxin Wang. Xiaoaiping injection affects the invasion and metastasis of hepatocellular carcinoma by controlling AFP expression. Oncol Transl Med, 2023, 9: 35-42. |
| 消癌平注射液通过控制AFP的表达影响肝细胞癌的侵袭和转移 |
| Xiaoaiping injection affects the invasion and metastasis of hepatocellular carcinoma by controlling AFP expression |
| Received:November 11, 2022 Revised:March 06, 2023 |
| DOI：10.1007/s10330-022-0616-6 |
| 中文关键词: 消癌平；注射液；AFP；HCC；侵袭；转移 |
| 英文关键词: Xiaoaiping injection; Alpha-fetoprotein (AFP); hepatocellular carcinoma (HCC); invasion, metastasis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Shu Huang | Department of Hepatology of Integrated Traditional Chinese and Western Medicine, The Third People''''s Hospital of Hubei Province affiliated with Jianghan University | huangshu__2020@163.com | | Ganxin Wang* | Department of Hepatology of Integrated Traditional Chinese and Western Medicine, The Third People''''s Hospital of Hubei Province affiliated with Jianghan University | Medicine_wgx@163.com |
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| 中文摘要: |
| 消癌平（XAP）是一种传统的中药，由于其高效低毒的特点，是临床上常用的抗癌药物。它能有效地抑制肝细胞癌（HCC）的生长。AFP作为肝细胞癌的关键诊断指标，与肝细胞癌的一些恶性细胞学行为密切相关。然而，AFP和消癌平注射液的表达是否与肝细胞癌的侵袭和转移有关，目前还不清楚。在本研究中，我们旨在评估消癌平对肝癌侵袭和转移的影响和内在机制。方法：采用细胞划痕法、Transwell技术和Western blot检测XAP处理组和空白对照组之间Hep3B细胞的不同侵袭和转移能力。比较不同AFP表达水平组的Hep3B细胞的侵袭和转移能力。采用AFP mRNA seq techenology分析了与AFP和消癌平相关的肿瘤侵袭和转移机制。结果：XAP组的细胞侵袭和转移能力明显低于对照组（P<0. 05）；与对照组相比，XAP处理后AFP的表达下降，差异有统计学意义（P<0. 05）。在AFP水平上调的情况下，Hep3B细胞的侵袭和转移能力得到促进，而在AFP沉默的情况下则受到抑制。XAP注射液和AFP通过影响基质金属蛋白酶（MMPs）蛋白来调节HCC的侵袭和转移能力。结论：XAP注射液通过影响AFP的表达来抑制HCC的侵袭和转移能力，而AFP的抑制是通过影响MMPs蛋白实现的。 |
| 英文摘要: |
| Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancer
drug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibit
the growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker and
is closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression and
XAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, we
aimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..
Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the different
invasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowed
comparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.
AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasis
associated with AFP and XAP treatment.
Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in the
control group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantly
decreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize was
promoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAP
injection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases
(MMPs).
Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expression
of AFP; additionally, this inhibition of AFP is achieved by affecting MMPs. |
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