| Qian Zhong. Mechanism of lncRNA SNHG19 miR-299-5p MAPK6 signaling axis promoting metastasis of non-small cell lung cancer cells. Oncol Transl Med, 2022, 8: 247-258. |
| Mechanism of lncRNA SNHG19 miR-299-5p MAPK6 signaling axis promoting metastasis of non-small cell lung cancer cells |
| Received:September 05, 2022 Revised:October 22, 2022 |
| View Full Text View/Add Comment Download reader |
| KeyWord:long noncoding RNA small nucleolar RNA host gene 19; MicroRNA-299-5p; non-small cell |
|
| Hits: 1982 |
| Download times: 2426 |
| Abstract: |
| Objective The aim of this study was to explore the mechanism behind lncRNA small nucleolar RNA host
gene 19 (lncRNA SNHG19)/microrNA-299-5P (miR-299-5p)/mitogen-activated protein kinase 6 (MAPK6)
signaling axis promoting metastasis of non-small cell lung cancer (NSCLC).
Methods To analyze the abnormal expression of lncRNAs in NSCLC, 50 surgically resected NSCLC and
adjacent tissue samples were collected from August 2021 to August 2022. The mRNA expression levels of
lncRNA SNHG19, Mir-299-5p, and MAPK6 were detected by qRT-PCR. The functions of lncRNA SNHG19,
Mir-299-5p and MAPK6 were investigated by CCK-8, clone formation, EdU, scratch, Transwell western
blotting (WB)and in vivo xenograft assay. RNA fluorescence in-situ hybridization (FISH), RNA pull-down,
dual luciferase reporter, and RNA co-immunoprecipitation assays were used to explore the mechanism of
action between lncRNA SNHG19, miR-299-5p, and MAPK6.
Results High expression of lncRNA SNHG19 was correlated with poor prognosis, tumor size, lymph node
metastasis, and TNM stage in NSCLC patients (P < 0.05). Cell function experiments showed that lncRNA
SNHG19 could improve the proliferation, clone formation, migration, and invasion ability of A549 cells both
in vitro and in vivo (all P < 0.05) and increased the relative expression levels of vimentin and MAPK6 (P <
0.05). The relative expression level of E-cadherin was decreased (P < 0.05). lncRNA SNHG19 can interact
with Mir-299-5p and regulate the expression level of MAPK6.
Conclusion lncRNA SNHG19 is upregulated in NSCLC tissues and cells, and its high expression is
associated with tumor progression and poor survival. Moreover, it can act as a molecular sponge for Mir-
299-5p to regulate MAPK6 expression and promote the proliferation and metastasis of A549 cells. |
| Close |
|
|
|