| Chengcong Liu,Guoxin Sun,Huizhe Wang,Gaishuang Shang,Xiong Yan,Xiao Zou. Analysis of the intestinal flora in patients with primary liver cancer. Oncol Transl Med, 2023, 9: 28-34. |
| 原发性肝癌患者肠道菌群分析 |
| Analysis of the intestinal flora in patients with primary liver cancer |
| Received:May 27, 2022 Revised:March 02, 2023 |
| DOI：10.1007/s10330-022-0575-5 |
| 中文关键词: 肝癌；肠道菌群；基因组测序 16s rDNA |
| 英文关键词: liver cancer; intestinal flora; genome sequencing; 16s rDNA |
| 基金项目:青岛市民生科技计划项目 |
| Author Name | Affiliation | E-mail | | Chengcong Liu | Department of Gastrointestinal Surgery, Qingdao Central Hospital | 84285161@163.com | | Guoxin Sun | Department of Gastrointestinal Surgery, Qingdao Central Hospital | | | Huizhe Wang | Department of Gastrointestinal Surgery, Qingdao Central Hospital | | | Gaishuang Shang | Department of Gastrointestinal Surgery, Qingdao Central Hospital | | | Xiong Yan | Department of Gastrointestinal Surgery, Qingdao Central Hospital | | | Xiao Zou* | Department of Gastrointestinal Surgery, Qingdao Central Hospital | 1392365491@qq.com |
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| 中文摘要: |
| 摘要：目的 探讨原发性肝癌患者肠道菌群较健康人肠道菌群的差异及差异菌群对肝癌发生发展的影响。方法 收集青岛市第六人民医院2019年1月～2020年12月接受系统完整治疗且临床资料完整的原发性肝癌患者77例，以同期健康体检人群26例作为健康对照。对入组患者及健康志愿者大便菌群进行16s rDNA高通量测序和宏基因组测序，利用LefSe生物信息学软件分析肠道菌群差异。结果 所有样本检测显示与健康人群对比，根据细菌分类学，门、纲、目、科、属、种有显著统计学差异的菌落数分别为5、5、5、8、11、14 （p<0.05）。结论 原发性肝癌患者较健康健康人群存在明显的肠道菌群的改变差异，肠道菌群改变可能与原发性肝癌的发生存在一定相关性，肠道菌群可能成为防治原发性肝癌的新靶点。 |
| 英文摘要: |
| Objective To investigate the differences in intestinal flora of patients with primary liver cancer and of
healthy individuals and to investigate the effect of the differential flora on the development of liver cancer.
Methods Overall, 67 patients with primary liver cancer who received systematic and complete treatment
between January 2019 and December 2020 at the Sixth People’s Hospital of Qingdao and had complete
clinical data were enrolled in this study, and 26 individuals who were healthy on physical examination in
the same period were used as healthy controls. Macro genome and 16s ribosome Deoxyribo Nucleic Acid
(rDNA) high-throughput sequencing were performed on the stool flora of the enrolled patients and controls,
and the differences in the intestinal flora were analyzed using the LEfSe bioinformatics software.
Results Compared with the control samples, all the tested patient samples showed statistically significant
differences in the number of colonies of 5 bacterial phyla, 5 orders, 8 families, 11 genera, and 14 species
(P < 0.05).
Conclusion Compared with healthy people, patients with primary liver cancer have significant differences
in the intestinal flora composition. The alteration of the intestinal flora may be correlated with the occurrence
of primary liver cancer, and the intestinal flora may become a novel target for the prevention and treatment
of primary liver cancer |
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