| Yulong Li,Yang Yang,Ruifang Sun. Expression and prediction of genes related to IGF2BP3 in gastric cancer. Oncol Transl Med, 2022, 8: 173-179. |
| IGF2BP3在胃癌中的表达及其相关基因的预测 |
| Expression and prediction of genes related to IGF2BP3 in gastric cancer |
| Received:January 14, 2022 Revised:August 15, 2022 |
| DOI：10.1007/s10330-022-0552-2 |
| 中文关键词: 胃癌，IGF2BP3, 生物信息学分析 |
| 英文关键词: gastric cancer (GC); IGF2 mRNA-binding protein 3 (IGF2BP3); bioinformatics analysis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Yulong Li* | Department of gastroenterology, Shaanxi Provincial People’s Hospital, Xi’an , China | liyulong0639@126.com | | Yang Yang | School of Public Health, Shaanxi University of Chinese Medicine | | | Ruifang Sun | Department of Pathology, School of Basic Medical Sciences, Xi''anJiaotong University Health Science Center, Xi''an Jiaotong University, Xi''an , China | |
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| 中文摘要: |
| 目的：胃癌是世界范围内最常见的高发病率和高死亡率的癌症之一。 IGF2BP3与多种肿瘤相关。本研究旨在分析IGF2BP3在胃癌中的表达并探讨与IGF2BP3相关的基因。
方法：应用生物信息学软件分析IGF2BP1、IGF2BP2、IGF2BP3等IGF2BPs家族成员在肿瘤中的表达情况，并分析GSE118897中IGF2BPs的表达情况。免疫组织化学检测胃癌样本中IGF2BP3的蛋白水平。通过cBioPortal检测 IGF2BP3 遗传特征的变化。应用LinkedOmics 探寻与IGF2BP3 相关的基因。
结果：Sangerbox显示所有IGF2BP家族成员在胃癌中均呈高表达。分析GSE118897 数据中 IGF2BP1、IGF2BP2 和 IGF2BP3 的表达，发现IGF2BP3 在 胃癌中呈高表达。免疫组化实验表明IGF2BP3 在胃癌组织中的表达高于癌旁组织。cBioPortal显示错义突变为IGF2BP3 突变的主要形式。最后，LinkedOmics 分析显示， PLAGL2、GET4、IGF2BP1、HMGA2、CLDN6、HOXC13、SMARCA2、TMEM66、CIRBP、NFIX、SLC25A12 和 CYB5D2等基因与IGF2BP3表达相关。
结论：我们的研究发现 IGF2BP3 在胃癌中高表达。此外，本研究分析了胃癌中与IGF2BP3相关的潜在基因。 |
| 英文摘要: |
| Objective Gastric cancer (GC) is one of the most prevalent cancers worldwide and is associated with
high morbidity and mortality rates. The IGF2 mRNA-binding protein (IGF2BP) participates in a variety of
cancers. The aim of this study was to analyze the expression of IGF2BP3 and explore the genes related
to IGF2BP3 in GC.
Methods Bioinformatics software was used to analyze the expression of IGF2BP1, IGF2BP2, and
IGF2BP3 in tumors, and the expression of IGF2BPs in the GSE118897 dataset. Immunohistochemistry
was performed to detect the protein level of IGF2BP3 in GC samples. cBioPortal was used to query gene
alteration of IGF2BP3. LinkedOmics was used to identify genes related to IGF2BP3.
Results Sangerbox analysis showed that the expression of all IGF2BP family members was higher in GC.
cBioporta analysis showed that gene alteration of IGF2BP3 in stomach adenocarcinoma included mutation
and amplificatio. LinkedOmics analysis showed that many genes were correlated with IGF2BP3, such as
PLAGL2, GET4, IGF2BP1, HMGA2, CLDN6, HOXC13, SMARCA2, TMEM66, CIRBP, NFIX, SLC25A12, and
CYB5D2.
Conclusion In this study, we founded that IGF2BP3 was overexpressed in GC. Furthermore, this study
identified potential genes related to IGF2BP3 in GC, which should be studied further. |
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