| Mmengting Dong,Jiayu Sheng,Chunyang Li,Patiguli·JIAPAER,Xiaofei Li,Minjia Yuan,Xiaohong Xue,Ke Jiang. CD14 macrophage and IL-10 levels in the. Oncol Transl Med, 2022, 8: 165-172. |
| 乳腺癌外周血CD14巨噬细胞与IL-10联合检测的诊断价值评估 |
| CD14 macrophage and IL-10 levels in the |
| Received:November 27, 2021 Revised:August 09, 2022 |
| DOI：10.1007/s10330-021-0539-9 |
| 中文关键词: 乳腺癌；巨噬细胞；IL-10；外周血；诊断价值 |
| 英文关键词: breast cancer; macrophage; IL-10; peripheral blood; diagnostic value |
| 基金项目:Supported by grants from the National Natural Science Foundation of China (No. 82104952, No. 82004240), Special project for clinical |
| Author Name | Affiliation | E-mail | | Mmengting Dong | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | nancyd33@163.com | | Jiayu Sheng | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Chunyang Li | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Patiguli·JIAPAER | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Xiaofei Li | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Minjia Yuan | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Xiaohong Xue | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Ke Jiang* | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | surgeonjk@163.com |
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| 中文摘要: |
| 【摘要】 目的 探讨乳腺癌外周血中CD14巨噬细胞与IL-10联合检测的诊断价值。 方法 采用流式细胞术检测乳腺癌患者与健康对照组的外周血CD14巨噬细胞水平，采用酶联免疫吸附法（ELISA）检测两组外周血IL-10表达水平，完成CD14巨噬细胞各组群与IL-10的联合检测模型建立，利用受试者工作特征( receiver operating characteristic , ROC) 曲线分析检验CD14巨噬细胞各组群、IL-10以及联合检测的诊断效能，并通过卡方检验得到以上指标与临床病理特征的关系。另选取乳腺良恶性患者检测外周血，采用同样的方法检测CD14巨噬细胞与IL-10水平，进一步验证联合检测模型的诊断效能。结果 乳腺癌患者CD14+巨噬细胞群及IL-10表达水平较高，与健康人群呈明显差异（P＜0.05），ROC曲线显示CD14+巨噬细胞与IL-10联合检测的曲线下面积（Area Under Curve，AUC）为0.830，敏感度为72.0%，特异度为87.5%，其诊断效能优于IL-10、CD14+、CD14-巨噬细胞的单独检测，以及IL-10与CD14-巨噬细胞及总巨噬细胞的联合检测结果。临床病理特征相关性分析显示CD14+巨噬细胞和IL-10联合检测与肿块大小、TNM分期、淋巴结情况、ER表达情况及Ki-67表达均明显相关（P＜0.05）。验证性分析与试验结果基本一致。 结论 外周血巨噬细胞可成为乳腺癌的独立诊断标志物，CD14+巨噬细胞与IL-10联合检测具有提示预后不良，指导BC发展及病情监测的无限潜能，为乳腺癌相关巨噬细胞研究提供新思路。 |
| 英文摘要: |
| Objective To explore the correlation between macrophages and interleukin-10 (IL-10 in the peripheral
blood of breast cancer (BC) patients and the diagnostic value of joint detection.
Methods BC patients (n = 50) and healthy controls (n = 40) were prospectively recruited. The percentage
of circulating cluster of differentiation 14 (CD 14) macrophage cells was analyzed by flow cytometry, and
an enzyme-linked immunosorbent assay (ELISA) was used to detect IL-10 expression levels. Receiver
operating characteristic (ROC) curves were used to verify the diagnostic value of the models based on the
expression of CD14 macrophage cell populations and IL-10. In addition, the association between model
expression and clinicopathological characteristics was investigated. Another 30 patients with BC and 30
with benign breast disease were selected to validate the IL-10 and CD14 macrophage joint detection model
using the same method.
Results CD14 macrophage and IL-10 expression levels in BC patients were higher than those in healthy
controls (P < 0.05). The ROC curve showed that the area under the curve (AUC) of CD14+ macrophages
combined with IL-10 was 0.830, the sensitivity was 72.0%, and the specificity was 87.5%. Its diagnostic
efficiency was better than all other single and joint detections. Correlation analysis of clinicopathological
features showed that IL-10 and CD14+ macrophage joint detection was significantly correlated with tumor
size, tumor-node-metastasis (TNM) stage, and lymph node, estrogen receptor (ER), and Ki-67 expression
(P < 0.05). The validation analysis results were consistent with the test results.
Conclusion Peripheral blood macrophages can be an independent diagnostic marker for BC. Joint
detection of CD14- macrophages and IL-10 suggests poor prognosis, which has unlimited potential to guide
BC development and provides a new theory for studying tumor-associated macrophages in BC. |
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