| Yaqiong Ni,Wei Yao,Yunsheng Wang,Hui Wang,Qinjiang Liu. Effects of rearranged during transfection mutation on calcitonin and procalcitonin expression in sporadic medullary thyroid carcinoma. Oncol Transl Med, 2022, 8: 121-125. |
| 散发型甲状腺髓样癌RET突变对CTn和PCT表达的影响 |
| Effects of rearranged during transfection mutation on calcitonin and procalcitonin expression in sporadic medullary thyroid carcinoma |
| Received:November 17, 2021 Revised:June 19, 2022 |
| DOI：10.1007/s10330-021-0536-6 |
| 中文关键词: 散发型甲状腺髓样癌；降钙素原；降钙素：RET |
| 英文关键词: sporadic medullary thyroid carcinoma (SMTC); procalcitonin; calcitonin: rearranged during transfection (RET) |
| 基金项目:甘肃省卫生行业科研计划项目 (NO.GSWSKY2018-13) |
| Author Name | Affiliation | E-mail | | Yaqiong Ni | Gansu provincial Cancer Hospital | 13919306776@163.com | | Wei Yao | Gansu provincial Cancer Hospital | | | Yunsheng Wang | Gansu provincial Cancer Hospital | | | Hui Wang | Gansu provincial Cancer Hospital | | | Qinjiang Liu* | Gansu provincial Cancer Hospital | liuqj99@126.com |
|
| Hits: 4431 |
| Download times: 5578 |
| 中文摘要: |
| 目的：探讨散发型甲状腺髓样癌（SMTC）RET突变对降钙素CTn和降钙素原（PCT）表达的影响。方法：采用PCR直接测序法检测SMTC（64例）RET突变，免疫组化SP法检测SMTC组织中CTn和PCT的表达水平。分析RET突变对CTn和PCT的表达的影响及与临床病理参数的的关系。结果：SMTC组织中CTn和PCT的表达率分别为90.6%（58/64）和67.2%（43/64）。CTn和PCT表达与患者性别、年龄和肿瘤包膜侵犯均无关（P>0.05），而与肿瘤大小和淋巴结转移有关（P<0.05）。CTn和PCT表达水平具有显著相关性（r =0.269，P =0.041），同时CTn阳性表达强度与RET突变呈正相关（r=0.507，P =0.000），而PCT表达与RET突变无关（r =0.188，P =0.136）。结论：CTn和PCT的高表达与髓样癌的病情进展有关，CTn表达强度与RET突变影响。PCT作为SMTC诊断及预后评估指标有一定价值。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the effects of rearranged during transfection (RET)
mutation on the expressions of calcitonin (CTn) and procalcitonin (PCT) in sporadic medullary thyroid
carcinoma (SMTC).
Methods RET mutation was detected by polymerase chain reaction direct sequencing in 64
cases of SMTC, and the expression levels of CTn and PCT in SMTC tissues were detected using the
immunohistochemical streptavidin-perosidase (SP) method. The effect of RET mutations on the expression
of CTn and PCT along with its relationship with clinicopathological parameters were analyzed.
Results The expression rates of CTn and PCT in SMTC tissues were 90.6% (58/64) and 67.2% (43/64),
respectively. CTn and PCT expression were found to be associated with tumor size and lymph node
metastasis (P < 0.05) but not with gender, age, or tumor capsule invasion (P > 0.05). There was a significant
correlation between CTn and PCT expression (r = 0.269, P = 0.041), and the intensity of positive CTn
expression was positively correlated with RET mutation (r = 0.507, P = 0.000). However, PCT expression
was not associated with RET mutation (r = 0.188, P = 0.136).
Conclusion High expression of CTn and PCT was associated with the progression of medullary
carcinoma, and the intensity of CTn expression was associated with RET mutation. PCT may provide
valuable information for the diagnosis and prognosis of SMTC. |
|
View Full Text
Download reader HTML全文 |
| Close |