| Huanhuan Zhao,Junyu Li,Yan Liu,Li Li. Association of 2-methoxyestradiol levels with the occurrence and development of endometrial cancer in humans. Oncol Transl Med, 2022, 8: 191-195. |
| 2-甲氧雌二醇与子宫内膜癌发生发展的关系 |
| Association of 2-methoxyestradiol levels with the occurrence and development of endometrial cancer in humans |
| Received:July 23, 2021 Revised:August 06, 2022 |
| DOI：10.1007/s10330-021-0510-0 |
| 中文关键词: 子宫内膜癌, 2-MeOE2, E2, 尿液, HPLC-MS |
| 英文关键词: endometrial cancer; 2-methoxyestradiol (2-MeOE2); estradiol (E2); urine; high-performance liquid chromatography-mass spectrometry (HPLC-MS) |
| 基金项目:Supported by the Hebei Province Medical Science Research Key Project (No. 20210276). |
| Author Name | Affiliation | E-mail | | Huanhuan Zhao | Department of Obstetrics and Gynecology, The Forth Hospital, Hebei Medical University | zhaohuanyikeda@126.com | | Junyu Li | Department of Obstetrics and Gynecology, The Forth Hospital, Hebei Medical University | | | Yan Liu | Department of Pharmacy, Hebei Medical University | | | Li Li* | Department of Obstetrics and Gynecology, The Forth Hospital, Hebei Medical University | lily_lucky1@163.com |
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| 中文摘要: |
| 目的：确定尿雌二醇（E2）和2-甲氧基雌二醇（2-MeOE2）水平与子宫内膜癌发生发展的关系。
方法：根据病例对照研究，收集28名绝经后子宫内膜癌患者24小时尿液标本，选择同地区28名绝经后健康女性对照的24小时尿液标本。2-MeOE2浓度检测使用中空纤维的液相色谱-质谱 (LC-MS) 方法液相微萃取 (HF-LPME)。 使用酶联免疫吸附试验 (ELISA) 检测 E2 的浓度。
结果：子宫内膜癌患者和对照组的雌激素水平不同。 病例组E2含量高于对照组（P<0.05）。 病例组2-MeOE2含量低于对照组（P < 0.05）。 病例组E2与2-MeOE2比值显着高于对照组（P < 0.05）。
结论：本研究结果表明，子宫内膜癌变过程中雌激素代谢物失衡，2-MeOE2 降低和 E2/2-MeOE2 比值升高可作为潜在的子宫内膜癌的生物标志物。。 |
| 英文摘要: |
| Objective The aim of the study was to determine the association of urinary levels of estradiol (E2) and
2-methoxyestradiol (2-MeOE2) with the occurrence and development of endometrial cancer.
Methods In this case-control study, 24-h urine specimens were collected from 28 postmenopausal
patients with endometrial cancer and 28 postmenopausal healthy female controls. The concentration of
2-MeOE2 was determined using liquid chromatography-mass spectrometry with hollow fiber liquid-phase
microextraction. The concentration of E2 was determined using an enzyme-linked immunosorbent assay.
Results Estrogen levels were different between the patients with endometrial cancer and controls. The
relative quantity of E2 in the case group was higher than that in the control group (P < 0.05), whereas that of
2-MeOE2 was lower in the case group than that in the control group (P < 0.05). The ratio of E2-to-2-MeOE2
in the case group was significantly higher than that in the control group (P < 0.05).
Conclusion The results of this study indicate an imbalance of estrogen metabolites in endometrial
carcinogenesis. Reduced 2-MeOE2 levels and elevated E2-to-2-MeOE2 ratio may be used as potential
biomarkers for the risk assessment of estrogen-induced endometrial cancer. |
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