| Bingdong Wang,Xin Liu,Yahong Bian,Guoxin Sun,Huizhe Wang,Jingjin Zhang,Zhengfu Zhang,Xiao Zou. Correlation analysis of breast fibroadenoma and the intestinal flora based on 16S rRNA sequencing. Oncol Transl Med, 2021, 7: 269-274. |
| 基于16S rRNA测序对乳腺纤维腺瘤与肠道菌群的相关性分析 |
| Correlation analysis of breast fibroadenoma and the intestinal flora based on 16S rRNA sequencing |
| Received:July 15, 2021 Revised:December 19, 2021 |
| DOI：10.1007/s10330-021-0509-9 |
| 中文关键词: 肠道菌群；雌激素；乳腺纤维腺瘤；16S核糖体RNA；高通量测序 |
| 英文关键词: intestinal flora; estrogen; breast fibroadenoma; 16S ribosomal RNA; high-throughput sequencing |
| 基金项目:青岛市2019年度医药科研指导计划（2019-WJZD140） |
| Author Name | Affiliation | E-mail | | Bingdong Wang | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | 1326889160@qq.com | | Xin Liu | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | | | Yahong Bian | Qingdao Central Hospital Affiliated to Qingdao University | | | Guoxin Sun | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | | | Huizhe Wang | Department of Breast Surgery, Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266042, China | | | Jingjin Zhang | Linyi Central Hospital, Linyi 276400, China | | | Zhengfu Zhang* | Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266003, China | sqzzf1964@163.com | | Xiao Zou | Department of Breast Surgery, Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266042, China | 1392365491@qq.com |
|
| Hits: 5397 |
| Download times: 6460 |
| 中文摘要: |
| 目的 通过16S核糖体RNA（rRNA）高通量测序分析乳腺纤维腺瘤患者的肠道菌群特征。
方法 运用高通量测序技术对随机收集的20例乳腺纤维腺瘤患者与36例健康受试者的粪便标本进行16S rRNA V4区测序。通过mothur（ v.1.39.5） 软件计算样品的Alpha多样性（Chao指数、shannon指数）。通过QIIME（ v1.80）进行Beta多样性分析。运用SPSS 23.0版软件进行数据分析，采用两独立样本T检验分析两组间菌群丰度差异。
结果 相对于健康对照组，乳腺纤维腺瘤患者的肠道菌群α多样性具有升高趋势，但并无统计学差异（P＞0.05）；在门水平，两组间2大门类存在统计学差异，乳腺纤维腺瘤组相对于健康对照组，厚壁菌门(Firmicutes)丰度更高（P＜0.05），健康对照组相对于乳腺纤维腺瘤组互养菌门（Synergistetes）丰度更高（P＜0.005）。在属水平，两组样本间共5大菌属存在统计学差异，乳腺纤维腺瘤组相对于健康对照组布劳特菌属（Blautia）（P＜0.005）、粪球菌属(Coprococcus)（P＜0.005）、罗氏菌属(Roseburia)（P＜0.05）、瘤胃球菌属(Ruminococcus)（P＜0.005）菌群丰度更高（P＜0.005），而健康对照组相对于乳腺纤维腺瘤组萨特氏菌属(Sutterella)（P＜0.05）菌群丰度更高。
结论 与健康对照组相比，乳腺纤维腺瘤患者的肠道菌群的多样性和丰度存在显著差异，表明肠道菌群失衡与乳腺纤维腺瘤的发生存在相关性。 |
| 英文摘要: |
| Objective To analyze the characteristics of the intestinal microflora in patients with breast fibroadenoma
using 16S ribosomal RNA (rRNA) high-throughput sequencing.
Methods Fecal samples from 20 patients with breast fibroadenoma and 36 healthy subjects were
randomly collected and analyzed using high-throughput sequencing technology for 16S rRNA V4 region
sequencing, and the alpha diversity (Chao index, Shannon index) was calculated using Mothur (v.1.39.5)
software. Beta diversity was analyzed using QIIME (v1.80). SPSS software (version 23.0) and the t-test
of two independent samples were used to analyze differences in the abundance of bacteria between the
two groups.
Results Compared with that in the healthy control group, the α diversity of the intestinal microflora in
breast fibroadenoma patients increased, but the difference was not statistically significant (P > 0.05). At the
phylum level, significant differences were observed between the two groups. The abundance of Firmicutes
was higher in the breast fibroadenoma group (P < 0.05), whereas the abundance of Synergistetes was
higher in the healthy control group (P < 0.005). A total of five bacterial genera showed significant differences
between the two groups: the breast fibroadenoma group showed higher levels of Bautia (P < 0.005),
Coprococcus (P < 0.005), Roseburia (P < 0.05), and Ruminococcus (P < 0.005), whereas Sutterella was
more abundant in the healthy control group than in the breast fibroadenoma group (P < 0.05).
Conclusion The diversity and abundance of the intestinal flora in patients with breast fibroadenoma are
significantly different from those in healthy subjects, suggesting that an imbalance in the intestinal flora is
correlated with the occurrence of breast fibroadenoma. |
|
View Full Text
Download reader HTML全文 |
| Close |
|
|
|