| Bingdong Wang,Xin Liu,Yahong Bian,Guoxin Sun,Huizhe Wang,Jingjin Zhang,Zhengfu Zhang,Xiao Zou. Correlation analysis of breast fibroadenoma and the intestinal flora based on 16S rRNA sequencing. Oncol Transl Med, 2021, 7: 269-274. |
| Correlation analysis of breast fibroadenoma and the intestinal flora based on 16S rRNA sequencing |
| Received:July 15, 2021 Revised:December 19, 2021 |
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| KeyWord:intestinal flora; estrogen; breast fibroadenoma; 16S ribosomal RNA; high-throughput sequencing |
| Author Name | Affiliation | E-mail | | Bingdong Wang | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | 1326889160@qq.com | | Xin Liu | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | | | Yahong Bian | Qingdao Central Hospital Affiliated to Qingdao University | | | Guoxin Sun | Department of breast surgery ,Qingdao university affiliated Qingdao central hospital | | | Huizhe Wang | Department of Breast Surgery, Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266042, China | | | Jingjin Zhang | Linyi Central Hospital, Linyi 276400, China | | | Zhengfu Zhang | Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266003, China | sqzzf1964@163.com | | Xiao Zou | Department of Breast Surgery, Qingdao Central Hospital AffiliatedBeginEttoEndETQingdao University, Qingdao 266042, China | 1392365491@qq.com |
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| Abstract: |
| Objective To analyze the characteristics of the intestinal microflora in patients with breast fibroadenoma
using 16S ribosomal RNA (rRNA) high-throughput sequencing.
Methods Fecal samples from 20 patients with breast fibroadenoma and 36 healthy subjects were
randomly collected and analyzed using high-throughput sequencing technology for 16S rRNA V4 region
sequencing, and the alpha diversity (Chao index, Shannon index) was calculated using Mothur (v.1.39.5)
software. Beta diversity was analyzed using QIIME (v1.80). SPSS software (version 23.0) and the t-test
of two independent samples were used to analyze differences in the abundance of bacteria between the
two groups.
Results Compared with that in the healthy control group, the α diversity of the intestinal microflora in
breast fibroadenoma patients increased, but the difference was not statistically significant (P > 0.05). At the
phylum level, significant differences were observed between the two groups. The abundance of Firmicutes
was higher in the breast fibroadenoma group (P < 0.05), whereas the abundance of Synergistetes was
higher in the healthy control group (P < 0.005). A total of five bacterial genera showed significant differences
between the two groups: the breast fibroadenoma group showed higher levels of Bautia (P < 0.005),
Coprococcus (P < 0.005), Roseburia (P < 0.05), and Ruminococcus (P < 0.005), whereas Sutterella was
more abundant in the healthy control group than in the breast fibroadenoma group (P < 0.05).
Conclusion The diversity and abundance of the intestinal flora in patients with breast fibroadenoma are
significantly different from those in healthy subjects, suggesting that an imbalance in the intestinal flora is
correlated with the occurrence of breast fibroadenoma. |
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