| Hua Yang,Yuxue Wei,Gangli Liu. Identification of microRNA-21 as a valuable diagnostic marker of oral squamous cell carcinoma and potential target. Oncol Transl Med, 2021, 7: 195-202. |
| microRNA-21作为口腔鳞状细胞癌的诊断价值评估及潜在靶点分析 |
| Identification of microRNA-21 as a valuable diagnostic marker of oral squamous cell carcinoma and potential target |
| Received:May 11, 2021 Revised:October 27, 2021 |
| DOI：10.1007/s10330-021-0494-4 |
| 中文关键词: microRNA-21作为口腔鳞状细胞癌的诊断价值评估及潜在靶点分析 |
| 英文关键词: miR-21; oral squamous cell carcinoma (OSCC); diagnostic meta-analysis; target gene prediction |
| 基金项目:National Natural Science Foundation of China（81402298） |
| Author Name | Affiliation | Postcode | | Hua Yang | Department of Stomatology, People''s Hospital of Lanling County | 277700 | | Yuxue Wei | Department of Stomatology, People''s Hospital of Lanling County | | | Gangli Liu* | Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, School of Stomatology, Qilu Medical College, Shandong University | 250012 |
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| 中文摘要: |
| 目的：总结TCGA数据库及文献中miR-21作为生物标志物在口腔鳞状细胞癌中的诊断价值并分析其潜在靶基因。方法：用生物信息学对TCGA数据库进行整理分析，确定miR-21在OSCC中表达量。 检索2021年3月之前在Embase、Pubmed/Medline、Scopus和Web of science发布的相关文献，按照纳入排除标准筛选文献，提取资料并进行质量评估。以双变量meta分析模型及ROC曲线平面图综合分析miR-21对口腔鳞状细胞癌的诊断价值。结果：共检出304篇相关文献，通过筛选7篇文献纳入Meta分析。 综合分析的结果如下：灵敏度0.76 [95％置信度区间（CI），0.57-0.88]；特异性0.77（95％CI，0.58-0.89）；正似然比3.34（95％CI，1.58-7.08）;负似然比0.31（95％CI，0.15-0.63）;诊断比值比10.75（95％CI，2.85-40.51）;SROC曲线下的面积0.83（95％CI，0.80-0.86）。Deeks漏斗图显示没有发表的偏倚（P = 0.54）。通过生物学网站预测miR-21潜在的靶基因，并用DAVID进行交集靶基因GO注释功能分析。结论：结果表明miR-21-3p和miR-21-5p较正常组织相比在OSCC组织中显著高表达（p<0.05），且meta分析结果表明其可以作为诊断OSCC的潜在生物标志物。此外，miR-21的58个潜在靶基因显著富集到28个GO注释功能通路，为进一步临床诊断价值研究提供了生物学基础。 |
| 英文摘要: |
| Objective The aim of the study was to summarize the diagnostic value of miR-21 as a biomarker in oral
squamous cell carcinoma (OSCC) using a review of the literature and data from the cancer genome atlas
(TCGA) database.
Methods Data from TCGA database was sorted and analyzed by bioinformatics to determine the
expression level of miR-21 in OSCC. Further, we searched for relevant articles in Embase, PubMed/
Medline, Scopus, and Web of Science published before March 2021, extracted the data, and conducted
quality assessment. The bivariate meta-analysis model with Stata 16.0 was used to analyze the diagnostic
value of miR-21 for OSCC.
Results A total of 304 related articles were identified, and seven were selected for meta-analysis. The
diagnostic results after analysis were as follows: sensitivity 0.76 [95% confidence interval (CI), 0.57–0.88];
specificity 0.77 (95% CI, 0.58–0.89); positive likelihood ratio 3.34 (95% CI, 1.58–7.08); negative likelihood
ratio 0.31 (95% CI, 0.15–0.63); diagnostic odds ratio 10.75 (95% CI, 2.85–40.51); and area under the curve
0.83 (95% CI, 0.80–0.86). The Deeks’ funnel chart showed that there was no potential bias (P = 0.54).
Prediction analysis of the potential target genes of miR-21 was performed via the biological website, and
DAVID was used to cross target genes for gene ontology (GO) annotation function analysis.
Conclusion The results showed that miR-21-3p and miR-21-5p were significantly more highly expressed
in OSCC tissues than in normal tissues (P < 0.05), and the results of the meta-analysis indicated that they
could be used as potential biomarkers in the diagnosis of OSCC. In addition, 58 potential target genes of
miR-21 were significantly enriched in 28 GO annotation functional pathways, which provided a biological
basis for further clinical diagnostic value research. |
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