| Tingting Hao,Chaoqi Wang,Yingjie Song,Wanyan Wu,Xuetao Li,Tao Fan. Relationship between miR-7-5p expression and 125I seed implantation efficacy in pancreatic cancer and functional analysis of target genes. Oncol Transl Med, 2021, 7: 177-182. |
| miR-7-5p表达与胰腺癌125I粒子植入疗效的关系及其靶基因功能分析 |
| Relationship between miR-7-5p expression and 125I seed implantation efficacy in pancreatic cancer and functional analysis of target genes |
| Received:May 07, 2021 Revised:August 23, 2021 |
| DOI：10.1007/s10330-021-0493-3 |
| 中文关键词: MiR-7-5p，胰腺癌，125I，放射性粒子植入 |
| 英文关键词: miR-7-5p; pancreatic cancer; 125I; radioactive seed implantation |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Tingting Hao | The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | 304150601@qq.com | | Chaoqi Wang | Department of Urinary Surgery, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028007, China | | | Yingjie Song | General Surgery, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Wanyan Wu | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Xuetao Li | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Tao Fan* | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | 1601340054@qq.com |
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| 中文摘要: |
| 目的 探讨胰腺癌组织中miR-7-5p的表达与组织间- 125i照射敏感性的关系，并分析靶基因的功能。
方法 收集37例不能切除的胰腺导管腺癌患者，采用放射性125I粒子植入治疗。采用RT-PCR检测肿瘤组织中miR-7-5p的表达水平，分析miR-7-5p表达与125I辐射敏感性的关系。利用生物信息学软件和在线工具预测miR-7-5p的靶基因，分析其功能注释和通路富集。
结果 125I粒子植入术后随访2个月。miR-7-5p高表达组的客观缓解率为65.0%(13/20)。miR-7-5p低表达组的客观缓解率为5.88% (1/17);两组间差异有统计学意义(χ2=13.654, P<0.001)。三个数据库共预测了187个目的基因。GO功能注释显示靶基因主要参与细胞对胰岛素刺激的反应;基因印迹调控基因表达的研究胞质;peptidyl-serine磷酸化;bHLH转录因子结合;装入囊泡的货物;细胞对肾上腺素刺激的反应;核浆等。KEGG通路富集显示靶基因主要参与ErbB信号通路;HIF-1信号通路;轴突的指导;长寿调控途径-多物种;内分泌阻力;神经胶质瘤;癌症中的胆碱代谢;EGFR酪氨酸激酶抑制剂耐药等。通过Cytoscape软件的MCODE分析发现，PIGH、RAF1、EGFR、NXT2、PIK3CD、PIK3R3、ERBB4、TRMT13和C5orf22是mir-7-5p靶基因聚类的关键模块。
结论 miR-7-5p在胰腺癌组织中的表达与125I粒子放射敏感性呈正相关。miR-7-5p通过靶向基因调控作用于PDAC的多个信号通路网络，参与其发生发展，有望成为PDAC患者125I粒子植入治疗敏感性的预测指标。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the relationship between miR-7-5p expression and
intertissue-125I irradiation sensitivity in pancreatic cancer tissues and to analyze the function of target genes.
Methods Thirty-seven patients with unresectable pancreatic ductal adenocarcinoma (PDAC) treated with
radioactive 125I seed implantation were enrolled. RT-PCR was used to detect the expression level of miR-7-
5p in cancer tissues and analyze the relationship between miR-7-5p expression and 125I radiation sensitivity.
Bioinformatic software and online tools were used to predict the miR-7-5p target genes and analyze their
functional annotation and pathway enrichment.
Results Radioactive 125I seed implantation was followed up for 2 months. The objective response rate of
the miR-7-5p high expression group was 65.0% (13/20), whereas the objective response rate of the miR-
7-5p low expression group was 5.88% (1/17), and the difference between the two groups was statistically
significant (χ2 = 13.654, P < 0.001). A total of 187 target genes were predicted using three databases.
GO functional annotation showed that target genes were mainly involved in cellular response to insulin
stimulus, regulation of gene expression by genetic imprinting, cytosol, peptidyl-serine phosphorylation,
bHLH transcription factor binding, cargo loading into vesicles, cellular response to epinephrine stimulus,
and nucleoplasm. KEGG pathway enrichment analysis showed that target genes were mainly involved
in the ErbB signaling pathway, HIF-1 signaling pathway, axon guidance, longevity regulatory pathway,
endocrine resistance, glioma, choline metabolism in cancer, and EGFR tyrosine kinase inhibitor drug
resistance. Molecular complex detection analysis by Cytoscape revealed that PIGH, RAF1, EGFR, NXT2,
PIK3CD, PIK3R3, ERBB4, TRMT13, and C5orf22 were the key modules of miR-7-5p target gene clustering.
Conclusion The expression of miR-7-5p in pancreatic cancer tissues positively correlated with the
radiosensitivity of 125I seeds. Via targeted gene regulation, miR-7-5p acts on the network of multiple
signaling pathways in PDAC and participates in its occurrence and development. Thus, miR-7-5p may
become a predictive index of 125I seed implantation therapy sensitivity in PDAC patients. |
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