| Xia Chen,Xiaoming Zhang,Xiangji Lu,Meng Ren,Rina Su,Weishi Gao,Yanwei Gao. Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer*. Oncol Transl Med, 2021, 7: 165-171. |
| 增强自体热休克蛋白70-肿瘤肽复合物对HER-3阳性乳腺癌治疗效果的研究 |
| Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer* |
| Received:February 24, 2021 Revised:July 29, 2021 |
| DOI：10.1007/s10330-021-0485-5 |
| 中文关键词: 热休克蛋白70-抗原肽复合物；Her-2蛋白；重组蛋白；树突状细胞；细胞免疫治疗 |
| 英文关键词: heat shock protein 70 peptide complexes (HSP70-PCs); HER-3 protein; recombinant protein; dendritic cells (DCs); cellular immunotherapy |
| 基金项目:乳腺癌HSP70-HER谱对诱导自体特异性CD8+NKT细胞活性的影响 |
| Author Name | Affiliation | Postcode | | Xia Chen | Inner Mongolia Red Cross Blood Center, Hohhot, Inner Mongolia 010010, China | 010010 | | Xiaoming Zhang | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Xiangji Lu | Inner Mongolia Armed Police Hospital, Hohhot, Inner Mongolia 010010, China | | | Meng Ren | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Rina Su | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Weishi Gao | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China * | | | Yanwei Gao* | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China * | 010017 |
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| 中文摘要: |
| 目的 探讨增强自体热休克蛋白70-肿瘤肽复合物对HER-3阳性乳腺癌患者治疗效果的方法。
方法 我们首先研究了HER-3在乳腺癌组织中的表达及其与患者肿瘤临床特征的关系。其次，我们从具有不同HER-2和HER-3表达的原发性乳腺癌细胞中纯化自体HSP70-PCs，并测定复合物诱导的自体树突状细胞和CD8+T细胞的抗肿瘤活性。再次，用重组人HSP70-HER-3蛋白复合物对从HER-3高表达乳腺癌细胞中纯化的自体HSP70-PCs进行干预，并检测其免疫活性。
结果 结果表明，HSP70-PCs与重组HSP70-HER-3蛋白复合物结合后，诱导自体CD8+T细胞杀伤HER-3阳性乳腺癌细胞的免疫活性明显强于自体HSP70-PCs。
结论 本研究为以HSP70-DC为基础的HER-3高表达乳腺癌患者的免疫治疗提供了新的方向。 |
| 英文摘要: |
| Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat
shock protein 70-peptide complexes (HSP70-PCs) on HER-3-overexpressing breast cancer.
Methods In this study, we first studied the expression of HER-3 in breast cancer tissues and its
relationship with patient characteristics. We then purified HSP70-PCs from primary breast cancer cells with
different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic
cells (DCs) and CD8+ T cells induced by these complexes. Third, recombinant human HSP70-HER-3
protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3–overexpressing
breast cancer cells, and the resulting immunological response was examined.
Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein
complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these
treatments induce autogenous CD8+ T cell killing of HER-3-positive breast cancer cells.
Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients
with HER-3-overexpressing breast cancer. |
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