| Xia Chen,Zhijian Dai,Xiangji Lu,Meng Ren,Zhenshan Guo,Yanwei Gao. Study on the antitumor effects of autologous and allogeneic CIK cells in patients with breast cancer. Oncol Transl Med, 2021, 7: 65-68. |
| Study on the antitumor effects of autologous and allogeneic CIK cells in patients with breast cancer |
| Received:February 24, 2021 Revised:May 04, 2021 |
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| KeyWord:CIK cells; autologous; allogeneic; cellular immunotherapy; breast cancer |
| Author Name | Affiliation | Postcode | | Xia Chen | Department of Apheresis, Inner Mongolia Red Cross Blood Center, Hohhot 010010, China | 010010 | | Zhijian Dai | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Xiangji Lu | Department of General Surgery, Inner Mongolia Armed Police Hospital, Hohhot 010010, China | | | Meng Ren | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Zhenshan Guo | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Yanwei Gao | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | 010017 |
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| Abstract: |
| Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cells
induced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells from
healthy adults.
Methods We used conventional methods to induce CIK cells originating from two peripheral blood
mononuclear cell types (from patients with breast cancer and healthy adults). Killing activity was detected
using an LDH assay, immunophenotypic changes were analyzed by flow cytometry, and the IFN-γ level of
culture supernatants was detected by ELISA.
Results The results showed that the proliferative capacity of the allogeneic CIK cells was significantly
higher than that of the autologous CIK cells. Compared with autologous CIK cells, the allogeneic CIK cells
had significantly enhanced anti-tumor activity against SKBR-3 cells (P < 0.01) and IFN-γ secretion (P <
0.05); moreover, they increased the ratio of CD3+ CD56+ cells and CD3+ CD8+ cells (P < 0.05).
Conclusion Healthy adult-derived induced CIK cells exhibited a stronger anti-tumor effect than induced
CIK cells derived from patients with breast cancer. The results of this study could provide experimental
evidence for the clinical application of CIK cells. |
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