| Xia Chen,Zhijian Dai,Xiangji Lu,Meng Ren,Zhenshan Guo,Yanwei Gao. Study on the antitumor effects of autologous and allogeneic CIK cells in patients with breast cancer. Oncol Transl Med, 2021, 7: 65-68. |
| 乳腺癌患者自体与异体CIK 细胞抗肿瘤效应的研究 |
| Study on the antitumor effects of autologous and allogeneic CIK cells in patients with breast cancer |
| Received:February 24, 2021 Revised:May 04, 2021 |
| DOI：10.1007/s10330-021-0483-3 |
| 中文关键词: CIK细胞；自体；异体；细胞免疫治疗 |
| 英文关键词: CIK cells; autologous; allogeneic; cellular immunotherapy; breast cancer |
| 基金项目:同种异体CIK细胞治疗恶性肿瘤的临床应用研究 |
| Author Name | Affiliation | Postcode | | Xia Chen | Department of Apheresis, Inner Mongolia Red Cross Blood Center, Hohhot 010010, China | 010010 | | Zhijian Dai | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Xiangji Lu | Department of General Surgery, Inner Mongolia Armed Police Hospital, Hohhot 010010, China | | | Meng Ren | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Zhenshan Guo | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | | | Yanwei Gao* | Department of Surgical Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, China | 010017 |
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| 中文摘要: |
| 目的 比较乳腺癌患者自体细胞因子诱导的杀伤细胞(cytokine -induced killer cells，CIK) 与异体（健康成人）CIK细胞的抗肿瘤效应。
方法 分别自乳腺癌患者及健康成人外周血单个核细胞诱导CIK 细胞，用流式细胞术分析免疫表型，ELISA 法测定其分泌干扰素-γ( IFN-γ)水平。同时应用乳酸脱氢酶法（LDH）检测两组CIK细胞对人乳腺癌细胞株SKBR-3的杀伤活性。
结果 异体CIK 细胞增殖能力明显高于自体CIK 细胞，CD3 + CD8 +、CD3 + CD56 +细胞比率较相同条件下自体CIK 细胞组显著增多( P＜0. 05)。培养第15d，异体CIK细胞分泌IFN-γ水平高于自体CIK细胞( P＜0. 05)，且对人乳腺癌细胞株SKBR-3细胞的杀伤率显著高于自体CIK 细胞( P＜0. 01)。
结论 健康成人CIK 细胞比乳腺癌患者的自体CIK 细胞有更强的抗肿瘤效应。 |
| 英文摘要: |
| Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cells
induced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells from
healthy adults.
Methods We used conventional methods to induce CIK cells originating from two peripheral blood
mononuclear cell types (from patients with breast cancer and healthy adults). Killing activity was detected
using an LDH assay, immunophenotypic changes were analyzed by flow cytometry, and the IFN-γ level of
culture supernatants was detected by ELISA.
Results The results showed that the proliferative capacity of the allogeneic CIK cells was significantly
higher than that of the autologous CIK cells. Compared with autologous CIK cells, the allogeneic CIK cells
had significantly enhanced anti-tumor activity against SKBR-3 cells (P < 0.01) and IFN-γ secretion (P <
0.05); moreover, they increased the ratio of CD3+ CD56+ cells and CD3+ CD8+ cells (P < 0.05).
Conclusion Healthy adult-derived induced CIK cells exhibited a stronger anti-tumor effect than induced
CIK cells derived from patients with breast cancer. The results of this study could provide experimental
evidence for the clinical application of CIK cells. |
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