| Chunlei Zhang,Dan Liu,Qinqin Tian,Qi Yang,Xiaoyuan Zi,Yinghao Sun. CircBAGE2 (hsa_circ_0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p ‘sponge’ to alter the proliferation and apoptosis of prostate cancer cells. Oncol Transl Med, 2021, 7: 221-228. |
| CircBAGE2（hsa_circ_0061259）通过吸附miR103a-3p调节CCND1与PDCD10表达来改变前列腺癌细胞的增殖和凋亡 |
| CircBAGE2 (hsa_circ_0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p ‘sponge’ to alter the proliferation and apoptosis of prostate cancer cells |
| Received:September 14, 2020 Revised:October 27, 2021 |
| DOI：10.1007/s10330-020-0454-4 |
| 中文关键词: 前列腺癌；circBAGE2；CCND1；PDCD10 |
| 英文关键词: prostate cancer (PCa); circBAGE2; CCND1; PDCD10 |
| 基金项目: |
| Author Name | Affiliation | Department | | Chunlei Zhang | Changhai Hospital, Navy Military Medical University | Department of Urology | | Dan Liu | Changhai Hospital, Navy Military Medical University | Department of Urology | | Qinqin Tian | Changhai Hospital, Navy Military Medical University | Department of Urology | | Qi Yang | Changhai Hospital, Navy Military Medical University | Department of Urology | | Xiaoyuan Zi | Changhai Hospital, Navy Military Medical University | Department of Urology | | Yinghao Sun* | Changhai Hospital, Navy Military Medical University | Department of Urology |
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| 中文摘要: |
| 目的：探索circBAGE2对前列腺癌细胞的影响和作用机制。
方法：通过RT-qPCR和sanger测序验证circBAGE2的测序结果。采用琼脂糖凝胶电泳检测GAPDH、BAGE2和circBAGE2对RNase R的抗性，以及它们在22RV1细胞的cDNA和gDNA中的表达情况。通过CCK8试验和流式细胞检测验证转染了circBAGE2干扰RNA的22RV1细胞的生物学功能变化。使用多个数据库来预测circRNA、miRNA和mRNA之间的结合位点。用蛋白质印迹法检测CCND1和PDCD10的表达。结果：与正常前列腺细胞系相比，circBAGE2在前列腺癌细胞系中高表达，且在癌组织中表达量高于癌旁组织。敲低circBAGE2表达量后，22RV1的增殖能力下降，凋亡增加。下调的circBAGE2降低了CCND1和PDCD10的表达。CircBAGE2与miR103a-3p具有结合位点，miR-103a-3p与CCND1和PDCD10的3"UTR匹配。
结论：CircBAGE2通过作为miR-103a-3p的“海绵”上调CCND1和PDCD10表达促进前列腺癌进展。CircBAGE2可能是前列腺癌的潜在治疗靶点和预测指标。 |
| 英文摘要: |
| Objective The aim of the article is to explore the function of circBAGE2 (hsa_circ_0061259) in prostate
cancer (PCa) cells.
Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR (qRT-PCR) and Sanger
sequencing. Agarose gel electrophoresis was used to detect the resistance of GAPDH, BAGE2, and
circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells. The biological functions of
circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs.
Multiple databases were used to predict the target binding sites between circRNAs, miRNAs, and mRNAs.
Western blotting was used to detect the expression of CCND1 and PDCD10.
Results CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that
in matched normal tissues and normal cells, and CircBAGE2 knockdown inhibits cell proliferation and
promotes apoptosis. Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10.
The 3’ UTRs of CCND1 and PDCD10 were matched by miR-103a-3p, which shared binding sites with
circBAGE2.
Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression
through its role as a ‘sponge’ of miR-103a-3p. CircBAGE2 may be a potential therapeutic target for PCa. |
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