Chunlei Zhang,Dan Liu,Qinqin Tian,Qi Yang,Xiaoyuan Zi,Yinghao Sun. CircBAGE2 (hsa_circ_0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p ‘sponge’ to alter the proliferation and apoptosis of prostate cancer cells. Oncol Transl Med, 2021, 7: 221-228. |
CircBAGE2 (hsa_circ_0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p ‘sponge’ to alter the proliferation and apoptosis of prostate cancer cells |
Received:September 14, 2020 Revised:October 27, 2021 |
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KeyWord:prostate cancer (PCa); circBAGE2; CCND1; PDCD10 |
Author Name | Affiliation | Department | Chunlei Zhang | Changhai Hospital, Navy Military Medical University | Department of Urology | Dan Liu | Changhai Hospital, Navy Military Medical University | Department of Urology | Qinqin Tian | Changhai Hospital, Navy Military Medical University | Department of Urology | Qi Yang | Changhai Hospital, Navy Military Medical University | Department of Urology | Xiaoyuan Zi | Changhai Hospital, Navy Military Medical University | Department of Urology | Yinghao Sun | Changhai Hospital, Navy Military Medical University | Department of Urology |
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Abstract: |
Objective The aim of the article is to explore the function of circBAGE2 (hsa_circ_0061259) in prostate
cancer (PCa) cells.
Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR (qRT-PCR) and Sanger
sequencing. Agarose gel electrophoresis was used to detect the resistance of GAPDH, BAGE2, and
circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells. The biological functions of
circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs.
Multiple databases were used to predict the target binding sites between circRNAs, miRNAs, and mRNAs.
Western blotting was used to detect the expression of CCND1 and PDCD10.
Results CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that
in matched normal tissues and normal cells, and CircBAGE2 knockdown inhibits cell proliferation and
promotes apoptosis. Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10.
The 3’ UTRs of CCND1 and PDCD10 were matched by miR-103a-3p, which shared binding sites with
circBAGE2.
Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression
through its role as a ‘sponge’ of miR-103a-3p. CircBAGE2 may be a potential therapeutic target for PCa. |
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