| Zhaoxia Luo,Xiuze Li,Shuhua Li,Bo Hou. A study of the potential adverse effects of electrosurgical smoke on medical staff during malignant tumor surgery. Oncol Transl Med, 2021, 7: 136-140. |
| 恶性肿瘤手术中的电外科烟雾对医护人员潜在危害的实验研究 |
| A study of the potential adverse effects of electrosurgical smoke on medical staff during malignant tumor surgery |
| Received:July 22, 2020 Revised:June 26, 2021 |
| DOI：10.1007/s10330-020-0447-7 |
| 中文关键词: 电外科；超声刀；恶性肿瘤；烟雾 |
| 英文关键词: electrosurgery; ultrasonic scalpel; malignant tumor; smoke |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Zhaoxia Luo | Operation Room, Mianyang Central Hospital | whx20212020@163.com | | Xiuze Li | Operation Room, Mianyang Central Hospital | | | Shuhua Li | Operation Room, Mianyang Central Hospital | | | Bo Hou* | Operation Room, Mianyang Central Hospital | hhh1471232021@163.com |
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| 中文摘要: |
| 目的：探讨恶性肿瘤手术中的电外科手术烟雾对医护人员潜在危害。方法：本研究分体外实验和体内实验两部分。体外实验：培养人甲状腺癌细胞系ARO并传代，吹达成单细胞悬液，调节细胞浓度约为2×106个/ml。用超声刀对培养基中肿瘤细胞进行灼烧，用Transwell?薄膜吸收手术烟雾；将捕获的颗粒用3ml培养基稀释，在37℃孵育箱中培养，显微镜下观察是否有细胞存活。并进行DNA基因分型分析，以确定手术烟雾中的细胞是否与原始肿瘤细胞一致。体内实验：利用balb/c小鼠构建甲状腺癌移植瘤模型，14d用超声刀对肿瘤组织进行解剖，利用Transwell膜系统收集电外科烟雾，收集完毕后将膜置于2ml漂洗液中洗脱，收集漂洗液，注射至10只小鼠的右侧腋下，察肿瘤的生长情况，处死后对肿瘤组织进行HE染色。结果：（1）对手术烟雾中细胞碎片进行培养，第1d即可看到活ARO细胞，在孵育第17d可见旺盛的细胞繁殖，几乎长满培养皿底。（2）DNA基因分型分析显示，膜系统收集的烟雾颗粒中的细胞与原始细胞的所有等位基因均相同的。表明烟雾中收集的细胞与ARO细胞在遗传上是相同的。（3）在接受烟雾颗粒漂洗液注射的10只小鼠中，有4只观察到了明显的肿瘤生长。HE染色显示，肿瘤组织中细胞核明显增大，大小不等、核染色质粗糙深染、核畸形多见、核与胞质比例失常，符合恶性肿瘤的一般形态学特征。结论：利用超声刀解剖肿瘤产生的手术烟雾中存在活的肿瘤细胞，并具有一定的生长活性，有必要对患者及医护人员进行相应的电外科烟雾防护。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the potential adverse effects of electrosurgical smoke
on medical staff performing malignant tumor surgery.
Methods This study was divided into two parts: in vitro and in vivo experiments. The human thyroid cancer
cell line, ARO, was cultured and passaged. The tumor cells were burned with an ultrasonic scalpel, and
the surgical smoke was absorbed by a transwell membrane. The captured particles were diluted in 3 mL
of culture medium, and cell survival was assessed under a microscope. DNA was extracted from the cells
for genotyping. BALB/c mice were used to construct thyroid cancer xenograft models. The tumor tissues
were dissected on day 14 using an ultrasonic scalpel. The smoke from the electrosurgical procedure was
collected on a transwell membrane. The membrane was washed in 2 mL of rinsing solution, and the solution
was then injected into the right armpit of 10 mice. After sacrifice, the tumor tissues were removed and
stained with hematoxylin and eosin (HE).
Results Viable ARO cells could be seen on the first day after culturing cell fragments from surgical
smoke, and vigorous cell proliferation could be seen on the 17th day of incubation. The genotype of the
cells cultured in the presence of smoke particles was identical to the genotype of the original cells. Tumor
growth was observed in four out of 10 mice injected with the smoke particle rinse. HE staining showed
a significantly increased number of nuclei in the tumor tissue, which was consistent with the general
morphological characteristics of malignant tumors.
Conclusion Viable tumor cells were detected in surgical smoke generated by ultrasonic scalpel
dissection, and these cells had growth activity. Thus, it is necessary to protect patients and medical staff
from electrosurgical smoke. |
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