Shengnan Zhao,Aixia Chen,Jingyu Cao,Zusen Wang,Weiyu Hu,Fei Zhou,Donghai Liang,Hongsheng Yu. Neurotrophin 3 hinders the growth and metastasis of hepatocellular carcinoma cells*. Oncol Transl Med, 2020, 6: 143-152.
Neurotrophin 3 hinders the growth and metastasis of hepatocellular carcinoma cells*
Received:May 08, 2020  Revised:May 08, 2020
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KeyWord:Hepatocellular carcinoma;Tumor progression;NTF3
Author NameAffiliationE-mail
Shengnan Zhao Department of Radiation Oncology, the Affiliated Hospital of Qingdao University, Qingdao 266000, China zsn0613@163.com 
Aixia Chen Department of Radiation Oncology, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Jingyu Cao Department of Hepatobilary and Pancreatic Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Zusen Wang Department of Hepatobilary and Pancreatic Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Weiyu Hu Department of Hepatobilary and Pancreatic Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Fei Zhou Department of Hepatobilary and Pancreatic Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Donghai Liang Department of Radiation Oncology, the Affiliated Hospital of Qingdao University, Qingdao 266000, China  
Hongsheng Yu Department of Radiation Oncology, the Affiliated Hospital of Qingdao University, Qingdao 266000, China qdhsyu@126.com 
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Abstract:
      Objective: Neurotrophin 3 (NTF3) is involved in numerous biological processes; however, its role in hepatocellular carcinoma (HCC) is not well examined. This study investigated NTF3 function in HCC progression and revealed the underlying molecular mechanisms. Methods: The prognostic relevance of NTF3 was determined by bioinformatical analysis of publicly available TCGA data. Immunohistochemistry of HCC biopsies were performed for exploring the expression of NTF3. Cell growth and proliferation were analyzed by Cell Counting Kit-8 (CCK-8) assay. Cell invasion and migration were analyzed by Boyden transwell and wound healing assays. Protein expression and mRNA levels were evaluated by immunoblotting and quantitative polymerase chain reaction (qPCR). Cell apoptosis was evaluated by flow cytometry. Results: NTF3 expression was overall lower in HCC tissues compared to adjacent non-tumor tissues. Lower NTF3 expression was significantly associated with decreased patient overall survival and lower NTF3 expression was significantly associated with clinicopathological features. NTF3 overexpression reduced the proliferation, migration, and invasion of HCC cell lines. Conclusions: Decreased expression of NTF3 is involved in poor prognosis of patients with HCC by promoting HCC cell proliferation, migration, and invasion. Our findings provided novel understanding into the pathogenesis of HCC and the role of NTF3 in tumor progression, suggesting that targeting NTF3 has potential therapeutic and diagnostic value for HCC treatment.
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