| LiuFeng,Jianxin Zhong,Jianbin Sun,Hailong Wu. Effects of MIF on proliferation, migration, and STAT1 pathway of colon cancer cells. Oncol Transl Med, 2020, 6: 121-125. |
| MIF对结肠癌细胞增殖、迁移及STAT1通路的影响 |
| Effects of MIF on proliferation, migration, and STAT1 pathway of colon cancer cells |
| Received:April 16, 2020 Revised:June 19, 2020 |
| DOI：10.1007/s10330-020-0419-9 |
| 中文关键词: 结肠癌；巨噬细胞移动抑制因子；信号转导及转录活化因子1；细胞增殖；细胞迁移 |
| 英文关键词: colon cancer; migration inhibitory factor; signal transducer and activator of transcription 1; cell proliferation; cell migration |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LiuFeng* | Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology | lf5806@sina.com | | Jianxin Zhong | Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology | | | Jianbin Sun | Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology | | | Hailong Wu | Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology | |
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| 中文摘要: |
| 目的：探究巨噬细胞移动抑制因子MIF对结肠癌细胞SW480增殖、迁移、信号转导及转录活化因子 (STATs) 1通路的影响。方法：使用siRNA 转染结肠癌SW480细胞系后敲低MIF， qRT -PCR及western blot检测结肠癌细胞中STAT1及CD74的mRNA和蛋白质的表达水平。通过transwell实验及MTT实验等检测结肠癌细胞的增殖与侵袭能力。利用免疫共沉淀技术检测处理组细胞及对照组细胞中CD74和STAT1蛋白相互作用。结果：细胞学实验示：敲低MIF后，结肠癌细胞的增殖和侵袭能力较对照组均下降，且有统计学差异（P<0.05）。免疫共沉淀实验结果显示：在结肠癌细胞系中敲低MIF可以抑制CD74和 STAT1蛋白的结合，两组对比有统计学差异（P<0.05）。结论：MIF可以促进CD74和STAT1蛋白结合从而影响结肠癌细胞的增殖迁移和侵袭能力。 |
| 英文摘要: |
| Objective This study aimed to investigate how macrophage migration inhibitory factor (MIF) regulates
the interaction of signal transducer and activator of transcription 1 (STAT1) with CD74, and affects colon
cancer proliferation and invasion.
Methods After transfecting MIF small interfering RNA into the SW480 cell line, the expression of STAT1
and CD74 mRNA was detected by qRT-PCR and western blotting. Transwell and MTT assays were
performed to detect the colon cancer cell invasion and proliferation ability. Co-immunoprecipitation was
used to detect the interaction between CD74 and STAT1 proteins in the treated and control groups.
Results The cellular biological assays (MTT and Transwell) showed that the proliferation and invasion
ability of colon cancer cells decreased after MIF knockdown; the results showed significant statistical
difference (P < 0.05). The results of the co-immunoprecipitation assay suggested that MIF knockdown in
colon cancer cells could inhibit the binding of CD74 and STAT1 proteins; statistical difference was observed
between the two groups (P < 0.05).
Conclusion MIF can increase the proliferation and invasion of colon cancer cells by promoting the
combination of CD74 and STAT1. |
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