Junyu Wu,Weijun Yuan,Yuhuan Wang,Xiaodong Zhao. Effect of miR-375 on non-small cell lung carcinoma invasion, migration, and proliferation through the CIP2A pathway. Oncol Transl Med, 2020, 6: 103-108. |
Effect of miR-375 on non-small cell lung carcinoma invasion, migration, and proliferation through the CIP2A pathway |
Received:January 16, 2020 Revised:June 20, 2020 |
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KeyWord:miR-375; invasion; migration; non-small cell lung cancer (NSCLC); CIP2A |
Author Name | Affiliation | E-mail | Junyu Wu | Department of Emergency, The Fourth Medical Center of General Hospital of Chinese People's Liberation Army, Beijing 100037, China | 474251924@qq.com | Weijun Yuan | Department of Special Medicine, School of Basic Medical College, Qingdao University, Qingdao 266021, China | | Yuhuan Wang | Department of Special Medicine, School of Basic Medical College, Qingdao University, Qingdao 266021, China | | Xiaodong Zhao | Department of Emergency, The Fourth Medical Center of General Hospital of Chinese People's Liberation Army, Beijing 100037, China | |
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Abstract: |
Objective The aim of this study was to study the effect of miR-375 on non-small cell lung carcinoma
(NSCLC) invasion, migration, and proliferation through the CIP2A pathway.
Methods We constructed a stable over-expressing cell line with lentivirus as the experimental group
(Lv-miR-375) and transfected the empty vector as the negative control group (Lv-NC). The expression level
of miR-375 was detected using real-time fluorescence quantitative PCR (qRT-PCR).Western blots were
used to detect the expression levels of cancerous inhibitor of PP2A (CIP2A), MYC, protein kinase B (AKT)
and p-AKT in Lv-NC- and Lv-miR-375-transfected cells. Transwell assays were conducted to detect the
cell invasion and metastasis ability, and the cell counting kit-8 (CCK8) was used to detect cell proliferation.
Results qRT-PCR showed that miR-375 was overexpressed in NSCLC. Compared to the Lv-NCtransfected cells, the western blot results showed that CIP2A, MYC and p-AKT were highly expressed in
Lv-miR-375-transfected cells. Transwell assays showed that the invasion and migration ability of Lv-miR-
375-transfected A549 cells was significantly higher than that of Lv-NC-transfected cells. CCK8 experiments
showed that compared to Lv-NC-transfected cells, the cell proliferation ability of the Lv-miR-375-transfected
cells increased.
Conclusion MiR-375 could promote the invasion, migration, and proliferation of NSCLC A549 cells via
the CIP2A pathway. MiR-375 is expected to become a new target for the treatment of NSCLC, and may
become an important biomarker for the diagnosis, prognosis, and treatment of the disease. |
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