| Yanwei Gao,Xia Chen,Weishi Gao,Xiangji Lu,Lin Peng. A study on melanoma treatment using dendritic cells loaded with antigens purified from melanoma cell lines. Oncol Transl Med, 2020, 6: 21-25. |
| 细胞株纯化肿瘤抗原负载树突状细胞治疗恶性黑色素瘤的研究 |
| A study on melanoma treatment using dendritic cells loaded with antigens purified from melanoma cell lines |
| Received:December 08, 2019 Revised:December 08, 2019 |
| DOI：10.1007/s10330-019-0396-6 |
| 中文关键词: 热休克蛋白70-肿瘤肽复合物，树突状细胞，CIK细胞，恶性黑色素瘤，细胞免疫治疗 |
| 英文关键词: heat shock protein 70 peptide complexes; dendritic cells; CIK cells; melanoma; cellular immunotherapy |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Yanwei Gao | Department of surgical oncology,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia | gaoyw0518@163.com | | Xia Chen | Department of blood components preparation,Inner Mongolia Red Cross Blood Center,Hohhot,Inner Mongolia | | | Weishi Gao | Department of surgical oncology,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia | | | Xiangji Lu | Department of emergency,Inner Mongolia Armed Police Hospital,Hohhot,Inner Mongolia | | | Lin Peng* | Department of gastroenterology, Inner Mongolia People’s Hospital | penglinlaoshi@126.com |
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| 中文摘要: |
| 目的：自人恶性黑色素瘤细胞株中分离纯化更有效的肿瘤肽复合物增强恶性黑色素瘤的治疗效果。
方法：自人恶性黑色素瘤细胞株（A375, A875, M21, M14, WM-35和SK-HEL-1）中分离纯化热休克蛋白70（Heat shock protein 70, HSP70）-肿瘤肽复合物，命名为“M-HSP70-PCs”，并检测其抗肿瘤活性。自9例恶性黑色素瘤患者肿瘤原代细胞中纯化的自体HSP70-肿瘤肽复合物作为对照。研究两种抗原肽复合物负载的树突状细胞抗原肿瘤的免疫效应。
结果：M-HSP70-PCs与黑色素瘤患者自体HSP70-抗原肽复合物具有相同的诱导DC成熟以及刺激DC-CIK细胞分泌IFN-γ的能力。同时，在对黑色素瘤患者原代细胞杀伤活性方面，两组间无显著性差异，且均明显强于无抗原负载的DC-CIK细胞。
结论：M-HSP70-PCs可能作为一种高效、泛化的肿瘤抗原应用于以DCs为基础的恶性黑色素瘤的治疗。 |
| 英文摘要: |
| Objective The aim of this study was to purify effective tumor peptide complexes from human melanoma
cell lines to enhance the treatment effects on melanoma.
Methods We purified heat shock protein 70 (HSP70)-peptide complexes (PCs) from human melanoma
cell lines A375, A875, M21, M14, WM-35, and SK-HEL-1. We named the purified product as M-HSP70-
PCs and determined its immunological activities. Autologous HSP70-PCs purified from primary tumor
cells of melanoma patients (9 cases) were used as controls. These two tumor antigenic complexes were
loaded into dendritic cells (DCs) and used to stimulate an antitumor response against tumor cells in the
corresponding patients.
Results Mature DCs pulsed with M-HSP70-PCs stimulated autologous T cells to secrete the same levels
of type I cytokines as the autologous HSP70-PCs. Moreover, DCs pulsed with M-HSP70-PCs endued CIK
cells with an equal ability as autologous HSP70-PCs to kill melanoma cells in the patients.
Conclusion M-HSP70-PCs may be used as an efficient and generalized tumor antigen in the treatment
of DC-based malignant melanoma. |
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