| Meng Ren,Huixia Xu,Xiangji Lu,Bingping Wang,Rina Su,Hao Zhang,Song Jiang,Fengying Gao,Yanwei Gao. The study of selective primary culture and determination of a breast cancer cell line in vitro. Oncol Transl Med, 2020, 6: 68-71. |
| The study of selective primary culture and determination of a breast cancer cell line in vitro |
| Received:September 02, 2019 Revised:April 21, 2020 |
| View Full Text View/Add Comment Download reader |
| KeyWord:breast cancer; primary culture; Trypan blue staining; hematoxylin and eosin (HE) staining |
| Author Name | Affiliation | E-mail | | Meng Ren | Inner Mongolia People’s Hospital | renm_2007@163.com | | Huixia Xu | Inner Mongolia People’s Hospital | | | Xiangji Lu | Armed Police General Hospital Inner Mongolia | | | Bingping Wang | Inner Mongolia People’s Hospital | | | Rina Su | Inner Mongolia People’s Hospital | | | Hao Zhang | Inner Mongolia People’s Hospital | | | Song Jiang | Inner Mongolia Medical University | | | Fengying Gao | Inner Mongolia Medical University | | | Yanwei Gao | Inner Mongolia People’s Hospital | gaoyw0518@163.com |
|
| Hits: 7118 |
| Download times: 7747 |
| Abstract: |
| Objective The successful establishment of a tumor cell bank is based on the premise that the target cells
can be cultured by a legitimate approach. In this experiment, we used primary culture to select and detect
breast cancer cells in vitro, which can provide experimental ideas and methods for the establishment of a
living tumor tissue cell bank.
Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who
needed surgical treatment in our hospital. Cells were isolated and used to establish successful cell culture.
Cell activity and cell purity were measured before liquid nitrogen cryopreservation.
Results (1) At the initial culture stage, cells grew with adherence. Cell multiplication could be seen after
the cell medium was exchanged three times. Cell viability was above 86%, while the viability of the target
cells was above 75%, as detected by hematoxylin and eosin (HE) staining. (2) The number of breast cancer
cells decreased, while the number of fibroblasts increased after five rounds of passage. (3) The success
rate was 73.08%, which did not include polluted cells and those that were not successfully cryopreserved.
Conclusion (1) breast cancer cells could be selected from primary culture in vitro through an appropriate
method. (2) Exchange of the cell medium and further cell passage improved cell multiplication. (3) The
experimental results could be monitored using trypan blue and HE staining. (4) The success of breast
cancer cell culture in vitro could be used as a reference for other cell culture, so as to establish a tumor
tissue cell bank. |
| Close |
|
|
|