| 闫坤,Hua Yan,Qin Zhou,Min Wan,YanYan Ge,Jin Lu. Expression and significance of PAX8 gene in ovarian cancer based on Oncomine database Meta-analysis. Oncol Transl Med, 2019, 5: 175-181. |
| 基于ONCOMINE数据库meta分析PAX8基因在卵巢癌中的表达及意义 |
| Expression and significance of PAX8 gene in ovarian cancer based on Oncomine database Meta-analysis |
| Received:June 03, 2019 Revised:September 18, 2019 |
| DOI：10.1007/s10330-019-0363-3 |
| 中文关键词: 卵巢癌；基因；配对盒基因8；癌症细胞系百科全书 |
| 英文关键词: ovarian cancer; gene; paired box 8; cancer cell line encyclopedia |
| 基金项目: |
| Author Name | Affiliation | E-mail | | 闫坤 | Department of Obstetrics and Gynecology, Bengbu Third People's Hospital, Bengbu 233000, China | 505821744@qq.com | | Hua Yan* | Department of Obstetrics and Gynecology, Bengbu Third People's Hospital, Bengbu 233000, China | 1532397489@qq.com | | Qin Zhou | Department of Obstetrics and Gynecology, Bengbu Third People's Hospital, Bengbu 233000, China | | | Min Wan | Department of Obstetrics and Gynecology, Bengbu Third People's Hospital, Bengbu 233000, China | | | YanYan Ge | Department of Anesthesiology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233003, China | | | Jin Lu | Anhui Key Laboratory of Tissue Transplantation, Bengbu 233000, China | |
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| 中文摘要: |
| 目的 研究PAX8基因在卵巢癌中的表达及意义。方法 利用BioGPS数据库分析[ [基金项目]1、蚌埠医学院自然科学基金面上项目（BYKY1758）
2、安徽省大学生创新训练项目(201810367072)
[第一作者]闫坤，男（1988-），汉族，住院医师，主要从事妇科肿瘤研究。E-mail:505821744@qq.com
] PAX8基因在正常人体组织中的表达；利用Oncomine数据库检索PAX8基因信息，并对数据库检索结果进行再分析，对其在卵巢癌中表达的意义作荟萃分析；利用Kaplan-Meier Plotter数据库对卵巢癌患者预后生存周期进行分析；利用癌症细胞系百科全书（CCLE）对PAX8基因进行细胞系分析。结果 BioGPS数据库分析结果显示PAX8在正常卵巢组织不表达或低表达；Oncomine数据库检索PAX8得到454项不同类型的结果，PAX8表达有统计学差异意义的结果有9项，其中PAX8表达增高的结果有5项，PAX8表达降低的结果有4项,共包括417例研究样本；利用CCLE对PAX8基因进行细胞系分析结果显示在卵巢癌中高表达，与利用Oncomine数据库检索PAX8在卵巢癌中高表达的结果一致；Kaplan-Meier Plotter数据库结果显示PAX8表达水平对患者的总生存时间有着显著影响（P=0.042）；与低表达组相比，PAX8高表达组卵巢癌患者的总生存时间显著降低（P<0.05）。结论 通过对Oncomine数据库基因芯片中有关卵巢癌肿瘤相关基因信息进行深入研究，得出PAX8在卵巢癌组织中高表达，且与卵巢癌患者预后生存周期相关，为临床基因靶向肿瘤治疗药物的研制提供重要依据。 |
| 英文摘要: |
| Objective Although great progress has been made in the diagnosis and treatment of ovarian cancer, this
disease is still the leading cause of death due to female reproductive system tumors. It has been reported
that the paired box 8 (PAX8) gene is involved in the occurrence and development of a variety of human
tumors. However, few researchers have investigated this phenomenon in detail.
Methods Here, the BioGPS database was used to analyze the expression of the PAX8 gene in normal
tissues. The Oncomine database was used to search for PAX8 gene information, and the findings were
analyzed via a meta-analysis with regard to the significance of this gene in ovarian cancer. The Kaplan-
Meier Plotter database was used to analyze the prognosis of patients with ovarian cancer. The Cancer Cell
Line Encyclopedia (CCLE) was used only for obtaining cell line analysis data regarding the PAX8 gene.
Results The relevant results of the BioGPS database analysis showed that PAX8 is not expressed or
under-expressed in normal ovarian tissues. Oncomine data showed 454 different results; there were 417
study samples in total, with 9 results showing a significant statistical difference in PAX8 expression, 5 of
which were related to high expression of PAX8 and 4 of which were related to low PAX8 expression. Cell
line analysis data of the PAX8 gene obtained from CCLE showed high expression in ovarian cancer, which
is consistent with the high expression of PAX8 in ovarian cancer research found using the Oncomine
database. The Kaplan-Meier Plotter database showed that the expression level of PAX8 had a significant
effect on the overall survival time of patients (P = 0.042). Compared with the low expression group, the
overall survival time of ovarian cancer patients in the high expression group of PAX8 was significantly low
(P < 0.05).
Conclusion Through an in-depth study of the gene information of ovarian cancer-related genes using
the gene chip data in the Oncomine database, it was concluded that PAX8 is highly expressed in ovarian
cancer tissues and directly correlates to the prognostic survival of ovarian cancer patients. These findings
provide an important basis for the development of clinical gene-targeted cancer therapeutic drugs. |
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