| Na Chen,Zhongyin Zhou. Analyzing proteins in colonic tissues from mice with ulcerative colitis using the iTRAQ technology. Oncol Transl Med, 2019, 5: 6-11. |
| Analyzing proteins in colonic tissues from mice with ulcerative colitis using the iTRAQ technology |
| Received:November 13, 2018 Revised:March 01, 2019 |
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| KeyWord:ulcerative colitis (UC); isobaric tags for relative and absolute quantitation (iTRAQ); colonic tissue; differentially expressed proteins (DEPs) |
| Author Name | Affiliation | E-mail | | Na Chen | Department of gastroenterology, Renmin hospital of Wuhan university | 614220735@qq.com | | Zhongyin Zhou | Department of gastroenterology, Renmin hospital of Wuhan university | 13871029766@163.com |
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| Abstract: |
| Objective The aim of the study was to investigate the expression of proteins in colonic tissues of mice
with ulcerative colitis (UC) by using isobaric tags for relative and absolute quantitation (iTRAQ), probe into
the pathogenesis of UC, and find potential biomarkers of UC.
Methods Forty C57 mice were randomly divided into the control and model groups (20 mice in each
group). The mice in the model group were administered dextran sulphate sodium (DSS) for 7 consecutive
days ad libitum to induce acute colitis, and the colon tissue was extracted on the 8th day after the successful
establishment of the UC model. Proteins were identified by the iTRAQ and tandem mass spectrometry
techniques,and the identified proteins were analyzed by bioinformatics.
Results A total of 4019 proteins were identified among the two groups. Among them, 317 significant
differentially expressed proteins (DEPs) were detected according to the screening criteria for selecting
DEPs, i.e. fold change ratios ≥ 1.5 or ≤ 0.67 and P-values < 0.05, of which 156 were upregulated and
161 were downregulated. In the Gene Ontology (GO) analysis, the DEPs were classified into 48 functional
categories, which contained biological process, cellular component, and molecular function. Based on the
317 DEPs, the KEGG pathway analysis identified 160 vital pathways.
Conclusion DEPs in colonic tissues of mice with UC were screened using the iTRAQ technique, which
laid a foundation for further studies regarding the pathogenesis of UC. |
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