| Haining Meng,Junyu Wu,Qiao Huang,Jiwen Ren,Jiawei Huang,Weijun Yuan,Xuekun He,Yuhuan Wang,Congxian Cui,Shengwei Xu,Ruowu Shen. The effects of miR-375 expression in NSCLC via the 14-3-3ζ/ERK/MYC pathway. Oncol Transl Med, 2018, 4: 196-202. |
| MiR-375通过14-3-3ζ/ERK/MYC通路对非小细胞肺癌的影响 |
| The effects of miR-375 expression in NSCLC via the 14-3-3ζ/ERK/MYC pathway |
| Received:August 06, 2018 Revised:November 26, 2018 |
| DOI：10.1007/s10330-018-0290-0 |
| 中文关键词: 非小细胞肺癌，miR-375，14-3-3ζ， ERK， MYC |
| 英文关键词: NSCLC; miR-375; 14-3-3ζ; ERK; MYC |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Haining Meng | Qingdao University | 1535891698@qq.com | | Junyu Wu | Qingdao University | | | Qiao Huang | Qingdao University | | | Jiwen Ren | Qingdao University | | | Jiawei Huang | Qingdao University | | | Weijun Yuan | Qingdao University | | | Xuekun He | Qingdao University | | | Yuhuan Wang | Qingdao University | | | Congxian Cui | Affiliated Hospital of Qingdao University | | | Shengwei Xu* | The third people’s hospital of Qingdao | | | Ruowu Shen* | Qingdao University | shenruowu@aliyun.com |
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| 中文摘要: |
| 研究表明microRNAs（miRNAs）在肺癌细胞的侵袭，转移，分化和凋亡中发挥重要作用。 其中，microRNA-375（miR-375）已在多种肿瘤中检测到，但其在非小细胞肺癌（NSCLC）中的作用仍不清楚。 因此，我们调节miR-375的表达，以探究其对NSCLC中14-3-3ζ-ERK / MYC途径的影响。 我们的实验结果表明，miR-375和14-3-3ζ在NSCLC中高表达，并且miR-375的过表达增加了NSCLC细胞的侵袭，转移和增殖能力，并降低了凋亡能力。 此外，随着miR-375的上调，14-3-3ζ，p-ERK和MYC的蛋白质表达水平增加。 总之，我们的研究结果表明miR-375通过14-3-3ζ/ ERK / MYC途径增加NSCLC的恶性潜能。 |
| 英文摘要: |
| Objective There are several reports that suggest a significant role played by microRNAs (miRNAs) in
cell invasion, metastasis, differentiation, and apoptosis in lung cancers. miR-375 is one such miRNA that
has been detected in a variety of tumors, but its specific activity in non-small cell lung cancer (NSCLC)
remains unclear.
Methods In this study, we regulated the expression of miR-375, to evaluate its influence on the 14-3-3ζ/
ERK/MYC pathway in NSCLC.
Results The results of our experiments suggest that miR-375 and 14-3-3ζ are highly expressed in
NSCLC, and the over-expression of miR-375 increases the invasive, metastatic, and proliferative ability and
decreases the apoptotic ability of NSCLC cells. In addition, protein expression levels of 14-3-3ζ, p-ERK,
and MYC increased following the overexpression of miR-375.
Conclusion Overall, our findings indicate that miR-375 increases the malignant potential of NSCLC via
the 14-3-3ζ/ERK/MYC pathway. |
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