| Fenhong Kang,Yaping Luo,WANG Yan-long. N-myc downstream-regulated gene 2 promotes cell proliferation of HO-8910 ovarian cancer. Oncol Transl Med, 2018, 4: 171-175. |
| N-myc downstream-regulated gene 2促进卵巢癌细胞HO-8910 的增殖 |
| N-myc downstream-regulated gene 2 promotes cell proliferation of HO-8910 ovarian cancer |
| Received:June 26, 2018 Revised:August 24, 2018 |
| DOI：10.1007/s10330-018-0282-2 |
| 中文关键词: NDRG2 卵巢癌 HO-8910细胞 顺铂 |
| 英文关键词: N-myc downstream-regulated gene 2 (NDRG2); ovarian cancer; HO-8910 cell; MTT; cisplatin |
| 基金项目: |
| Author Name | Affiliation | Postcode | | Fenhong Kang | Department of Gynecology, Xiamen Maternal and Child Health Hospital, Xiamen 361003, China | 361003 | | Yaping Luo* | Department of Gynecology,Xiamen Maternal and Child Health Hospital,Xiamen | 361003 | | WANG Yan-long | Department of Gynecology,Xiamen Maternal and Child Health Hospital,Xiamen 361003, China | |
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| 中文摘要: |
| 目的：研究NDRG-2在卵巢癌中的表达情况及其在卵巢癌诊断和治疗方面的潜在用途。
方法：通过RT-PCR获得NDRG-2l/S基因片段并将其插入真核表达载体LNCX2，然后转染入HO-8910卵巢癌细胞；使用MTT及流式细胞仪监测转染pLNCX2-NDRG2 L/S质粒后HO-8910细胞的增殖情况；转染pLNCX2-NDRG2 L/S质粒后HO-8910细胞培养液中添加不同浓度顺铂并用流式细胞仪监测细胞的增殖情况；裸小鼠皮下注射转染转染pLNCX2-NDRG2 L/S质粒后HO-8910细胞成瘤，观察成立情况。结果：成功构建pLNCX2-NDRG2 L/S真核表达载体，NDRG2的表达与HO-8910卵巢癌细胞的增殖呈正相关；NDRG2 L/S促进了裸小鼠皮下卵巢癌的发生发展。结论：目前研究证实NDRG2 L/S基因促进了卵巢癌细胞的增殖，同时还增强了顺铂的耐受作用。 |
| 英文摘要: |
| Objective To investigate N-myc downstream-regulated gene 2 (NDRG2) expression in ovarian cancer
cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention.
Methods Human NDRG2L/S gene was obtained by revers-transcription polymerase chain reaction
(RT-PCR). Sequence analysis confirmed the identity of NDRG2L/S gene, which was then inserted into
a eukaryotic vector pLNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells.
Flow cytometry (FCM) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay
were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells
transfected with pLNCX2-NDRG2L/S was evaluated by FCM. Tumors were generated in female nude mice
by subcutaneous injection of HO-8910 cells.
Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2
expression positively correlated with the proliferation of HO-8910 cells. NDRG2L/S promoted tumorigenicity
in HO-8910 cells.
Conclusion The present study identified a novel function of NDRG2L/S gene and demonstrated its
involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in
HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation
and ovarian cancer progression. |
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