| Fenhong Kang,Yaping Luo,WANG Yan-long. N-myc downstream-regulated gene 2 promotes cell proliferation of HO-8910 ovarian cancer. Oncol Transl Med, 2018, 4: 171-175. |
| N-myc downstream-regulated gene 2 promotes cell proliferation of HO-8910 ovarian cancer |
| Received:June 26, 2018 Revised:August 24, 2018 |
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| KeyWord:N-myc downstream-regulated gene 2 (NDRG2); ovarian cancer; HO-8910 cell; MTT; cisplatin |
| Author Name | Affiliation | Postcode | | Fenhong Kang | Department of Gynecology, Xiamen Maternal and Child Health Hospital, Xiamen 361003, China | 361003 | | Yaping Luo | Department of Gynecology,Xiamen Maternal and Child Health Hospital,Xiamen | 361003 | | WANG Yan-long | Department of Gynecology,Xiamen Maternal and Child Health Hospital,Xiamen 361003, China | |
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| Abstract: |
| Objective To investigate N-myc downstream-regulated gene 2 (NDRG2) expression in ovarian cancer
cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention.
Methods Human NDRG2L/S gene was obtained by revers-transcription polymerase chain reaction
(RT-PCR). Sequence analysis confirmed the identity of NDRG2L/S gene, which was then inserted into
a eukaryotic vector pLNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells.
Flow cytometry (FCM) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay
were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells
transfected with pLNCX2-NDRG2L/S was evaluated by FCM. Tumors were generated in female nude mice
by subcutaneous injection of HO-8910 cells.
Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2
expression positively correlated with the proliferation of HO-8910 cells. NDRG2L/S promoted tumorigenicity
in HO-8910 cells.
Conclusion The present study identified a novel function of NDRG2L/S gene and demonstrated its
involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in
HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation
and ovarian cancer progression. |
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