| wei chen. Effects of Nordy on the proliferation, differentiation, and apoptosis of HPV16 subgene-immortalized human endocervical cells. Oncol Transl Med, 2018, 4: 18-25. |
| 诺帝对HPV-16亚基因永生化人宫颈上皮细胞增殖，凋亡和分化的影响 |
| Effects of Nordy on the proliferation, differentiation, and apoptosis of HPV16 subgene-immortalized human endocervical cells |
| Received:December 20, 2017 Revised:January 29, 2018 |
| DOI：10.1007/s10330-017-0248-8 |
| 中文关键词: 诱导分化；宫颈；永生化；HPV |
| 英文关键词: induced differentiation; cervical cancer; immortalization; HPV |
| 基金项目: |
| Author Name | Affiliation | E-mail | | wei chen* | the Sixth People Hospital of Chongqing, | trade468@126.com |
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| 中文摘要: |
| [摘要] 目的：研究诺帝（Nordy）对HPV16型亚基因(E6、E7)永生化人宫颈上皮细胞（H8细胞）增殖，分化和凋亡的影响。方法：MTT法检测诺帝对H8细胞增殖的抑制作用，免疫细胞化学SP法检测增殖细胞核抗原mcm5表达，流式细胞术（FCM）分析细胞周期和凋亡，光镜、电镜观察细胞形态变化， TRAP-ELISA法检测端粒酶活性变化。结果：10μmmol/L ~100μmmol/L浓度的诺帝能够不同程度地抑制H8细胞增殖；细胞核内mcm5的表达明显下降；可阻滞细胞周期于G0/G1期，S期细胞减少；细胞凋亡率增多,细胞形态朝良性方向分化, 端粒酶活性明显降低。结论：诺帝具有抑制H8细胞增殖, 促进凋亡，诱导其分化作用, 这种作用可能是通过阻滞细胞周期，降低端粒酶活性来实现的。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the effects of Nordy on the proliferation, differentiation,
and apoptosis of HPV16 subgene-immortalized human endocervical cells (H8 cells).
Methods After treatment with Nordy, H8 cell proliferation was evaluated using the MTT assay. The effects
of Nordy on the cell cycle and apoptosis of H8 cells were analyzed by flow cytometry (FCM) and the Annexin
V-FITC method. H8 cell MCM5 expression was detected by immunocytochemistry. Morphological changes
were observed by light and electron microscopy. Telomerase activity was evaluated by TRAP-ELISA.
Results We found that 10 μmol/L–100 μmol/L Nordy significantly inhibited H8 cell proliferation. After
treatment with Nordy, H8 cells were blocked in the G0/G1 phase, and the rate of cell apoptosis increased
significantly. Cells differentiated toward innocuousness, and MCM5 expression and telomerase activity
notably decreased.
Conclusion Nordy was observed to inhibit proliferation and promote apoptosis in H8 cells. Nordy also
induced H8 cell differentiation; this effect may have been achieved by blocking the cell cycle and decreasing
telomerase activity. |
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