| Xiaofeng Hou,Qinjiang Liu,Youxin Tian. Effect of parathyroid hormone on apoptosis of human medullary thyroid carcinoma cells. Oncol Transl Med, 2017, 3: 241-244. |
| Effect of parathyroid hormone on apoptosis of human medullary thyroid carcinoma cells |
| Received:May 02, 2017 Revised:December 21, 2017 |
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| KeyWord:parathyroid hormone (PTH); medullary thyroid carcinoma (MTC); cell apoptosis |
| Author Name | Affiliation | Postcode | | Xiaofeng Hou | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730050, China | 730050 | | Qinjiang Liu | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730050, China | 730050 | | Youxin Tian | Department of Head and Neck Surgery,The Cancer Hospital of Gansu Province | |
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| Abstract: |
| Objective The aim of the study was to investigate the effect of parathyroid hormone (PTH) on the
apoptosis of human medullary thyroid carcinoma (MTC) cells.
Methods In vitro cultured medullary thyroid carcinoma cell lines were treated with parathyroid hormone
and parathyroid hormone receptor-monoclonal antibody, and the apoptosis of cells was detected by flow
cytometry.
Results The cell morphology changed significantly after treatment based on the observation using the
inverted phase-contrast microscope. Various concentrations of parathyroid hormone and parathyroid
hormone receptor-monoclonal antibody effectively induced apoptosis in a time- and concentrationdependent
manner. When the concentration of parathyroid hormone was 2.0 μmol/L and that of parathyroid
hormone receptor-monoclonal antibody was 1.0 μmol/L, the apoptotic rate was 13.24% and 20.78%,
respectively, representing a statistically significant difference from that of the control cells (P < 0.05).
Conclusion PTH plays a role in inducing apoptosis of human MTC cells. |
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