| Xiaofeng Hou,Qinjiang Liu,Youxin Tian. Effect of parathyroid hormone on apoptosis of human medullary thyroid carcinoma cells. Oncol Transl Med, 2017, 3: 241-244. |
| 甲状旁腺素对人甲状腺髓样癌细胞凋亡作用的影响 |
| Effect of parathyroid hormone on apoptosis of human medullary thyroid carcinoma cells |
| Received:May 02, 2017 Revised:December 21, 2017 |
| DOI：10.1007/s10330-017-0227-7 |
| 中文关键词: 甲状旁腺素 甲状腺髓样癌 细胞凋亡 |
| 英文关键词: parathyroid hormone (PTH); medullary thyroid carcinoma (MTC); cell apoptosis |
| 基金项目:兰州市科技计划项目（2013-3-38） |
| Author Name | Affiliation | Postcode | | Xiaofeng Hou | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730050, China | 730050 | | Qinjiang Liu* | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730050, China | 730050 | | Youxin Tian | Department of Head and Neck Surgery,The Cancer Hospital of Gansu Province | |
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| 中文摘要: |
| 【摘要】 目的 探讨甲状旁腺素对人甲状腺髓样癌细胞体外凋亡作用的影响。方法 体外培养甲状腺髓样癌细胞株，经甲状旁腺素和甲状旁腺素受体单抗干预处理后流式细胞仪检测细胞凋亡。结果 倒置相差显微镜下细胞变化明显，各浓度甲状旁腺素和甲状旁腺素受体单抗均分别能有效地诱导细胞凋亡，凋亡作用呈时间和浓度依赖。当甲状旁腺素浓度为2.0μmol/L、甲状旁腺素受体单抗浓度为1.0μmol/L时，对细胞凋亡作用显著（P＜0.05），凋亡率分别为13.24%及20.78%。结论 甲状旁腺素在诱导细胞凋亡中能发挥了一定的作用。 |
| 英文摘要: |
| Objective The aim of the study was to investigate the effect of parathyroid hormone (PTH) on the
apoptosis of human medullary thyroid carcinoma (MTC) cells.
Methods In vitro cultured medullary thyroid carcinoma cell lines were treated with parathyroid hormone
and parathyroid hormone receptor-monoclonal antibody, and the apoptosis of cells was detected by flow
cytometry.
Results The cell morphology changed significantly after treatment based on the observation using the
inverted phase-contrast microscope. Various concentrations of parathyroid hormone and parathyroid
hormone receptor-monoclonal antibody effectively induced apoptosis in a time- and concentrationdependent
manner. When the concentration of parathyroid hormone was 2.0 μmol/L and that of parathyroid
hormone receptor-monoclonal antibody was 1.0 μmol/L, the apoptotic rate was 13.24% and 20.78%,
respectively, representing a statistically significant difference from that of the control cells (P < 0.05).
Conclusion PTH plays a role in inducing apoptosis of human MTC cells. |
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