| Lu Han,Yang-Yang Shen,Wen-Hua Zhao,Bao-Yong Sun,Xin Zhang,Kai Cui,Lei Zhou,Sheng Li. Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis. Oncol Transl Med, 2017, 3: 254-259. |
| CXCL12-CXCR4生物学轴在骨肉瘤中的表达及与血管生成相关性的研究 |
| Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis |
| Received:December 05, 2016 Revised:December 13, 2017 |
| DOI：10.1007/s10330-016-0211-1 |
| 中文关键词: 骨肉瘤; CXCL12-CXCR4生物学轴；血管生成；肺转移 |
| 英文关键词: osteosarcoma; stromal cell-derived factor-1 (CXCL12); chemokine receptors-4 (CXCR4) angiogenesis; pulmonary metastatics |
| 基金项目: |
| Author Name | Affiliation | Postcode | | Lu Han | School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250117, China | 250117 | | Yang-Yang Shen | Department of Anesthesiology, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Wen-Hua Zhao | Department of Tumor Minimally Invasive Surgery, Qianfo Hill Hospital of Shandong Province, Jinan 250014, China | | | Bao-Yong Sun | Department of Bone & Soft Tissue Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Xin Zhang | Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University | | | Kai Cui | Department of Hepatobiliary Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Lei Zhou | Department of Bone & Soft Tissue Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | cc | | Sheng Li* | School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250117, China | |
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| 中文摘要: |
| 【摘要】 目的 探讨CXCL12-CXCR4生物学轴在骨肉瘤中的表达及其在骨肉瘤血管生成中的作用。方法 应用免疫组化和实时荧光定量PCR技术检测40例骨肉瘤组织中CXCL12、CXCR4的表达情况。免疫组织化学法检测骨肉瘤中CD34的表达情况，形态计量学图象分析法测定微血管密度（microvessel density, MVD），分析CXCL12、CXCR4的表达与骨肉瘤MVD之间的关系以及与肺转移的关系。结果CXCL12蛋白在骨肉瘤组织中阳性率为40.0％（16/40）；CXCR4蛋白在骨肉瘤组织阳性率为60.0％（24/40）；实时荧光定量PCR也证实此结果结果。发生肺转移的患者骨肉瘤组织中CXCR4蛋白表达明显高于未发生转移者（P<0.05）。发生肺转移的患者骨肉瘤组织中MVD显著高于未转移者（P<0.01）。结论CXCR4的表达与骨肉瘤肺转移密切相关，其高表达对骨肉瘤中微血管的生成可能起促进作用。 |
| 英文摘要: |
| Objective: The expression of CXCL12 (stromal cell-derived factor-1)-CXCR4 (chemokine receptors-4) in
osteosarcoma and its role in angiogenesis were examined.
Methods: The expression of CXCR4 and CXCL12 in 40 cases of osteosarcoma was detected by
immunohistochemistry and real-time fluorescence quantitative PCR. The expression of CD34 in
osteosarcoma was detected by immunohistochemistry. Morphometric image analysis was performed
to measure microvessel density (MVD). Additionally, the relationship between CXCL12 and CXCR4
expression and MVD of osteosarcoma and pulmonary metastasis were analyzed.
Results: The positive rates of CXCL12 and CXCR4 protein expression in osteosarcoma were 40.0%
(16/40) and 60.0% (24/40), respectively. Fluorescence quantitative real-time PCR indicated that the
expression level of CXCR4 mRNA in pulmonary metastatic osteosarcoma was higher than that in nonpulmonary
metastatic osteosarcoma (P < 0.01). The level of MVD in pulmonary metastatic osteosarcoma
was higher than that in non-pulmonary metastatic osteosarcoma (P < 0.01).
Conclusion: The expression level of CXCR4 was significantly associated with pulmonary metastasis and
angiogenesis of osteosarcoma. |
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