| Chanli Zheng,Ling Xu,Yanjun Xie,Dongmei He,Yangqiu Li. Expression pattern of BIM, BCL-6, and c-MYC in adult B-cell acute lymphoblastic leukemia. Oncol Transl Med, 2017, 3: 151-155. |
| 成人急性B淋巴细胞性白血病中Bim，Bcl-6和c-Myc的表达谱 |
| Expression pattern of BIM, BCL-6, and c-MYC in adult B-cell acute lymphoblastic leukemia |
| Received:November 16, 2016 Revised:July 25, 2017 |
| DOI：10.1007/s10330-016-0204-4 |
| 中文关键词: Bim；Bcl-6；c-Myc；B细胞；急性淋巴细胞白血病；实时定量逆转录-聚合酶链式反应（qRT-PCR） |
| 英文关键词: BIM; BCL-6; c-MYC; B cell; acute lymphoblastic leukemia (ALL); quantitative reversetranscription polymerase chain reaction (qRT-PCR) |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Chanli Zheng | Jinan University | xiaoliergirl@126.com | | Ling Xu | Jinan University | | | Yanjun Xie | Panyu District Zhongcun Hospital | | | Dongmei He* | Jinan University | thedm@jnu.edu.cn | | Yangqiu Li | Jinan University | |
|
| Hits: 10035 |
| Download times: 11628 |
| 中文摘要: |
| 目的：研究初诊的成人急性B淋巴细胞性白血病（B cell acute lymphoblastic leukemia ，B-ALL）Bim，Bcl-6和c-Myc基因的表达谱。方法：利用实时定量逆转录-聚合酶链式反应，用SYBR绿色染料检测B-ALL病人血液中单核细胞Bim，Bcl-6和c-Myc基因mRNA的相对表达水平。健康成人外周血单核细胞作为对照。甘油醛-3-磷酸脱氢酶（Glyceraldehyde-3-phosphate dehydrogenase，Gapdh）作为内参照。结果：B-ALL病人Bim，Bcl-6和c-Myc的mRNA相对表达水平较健康人低，差异具有统计学意义(P<0.05)。初诊的B-ALL病人和获得完全缓解（complete remission,CR）的B-ALL病人Bim，Bcl-6和c-Myc的mRNA相对表达水平与健康人相比都降低，差异具有统计学意义(P<0.05)。而初诊的和获得CR的B-ALL病人Bim，Bcl-6和c-Myc的mRNA相对表达水平不具有统计学差异(P>0.05)。Spearman’s等级相关分析显示B-ALL病人的Bim表达水平与Bcl-6表达水平具有相关性。结论：在成人B-ALL中Bim，Bcl-6和c-Myc的mRNA相对表达水平降低。初诊的和获得CR的B-ALL病人Bim，Bcl-6和c-Myc的表达谱可能是相似的。Bim，Bcl-6和c-Myc基因表达下调的特点可能作为诊断成人B-ALL的标志物之一。 |
| 英文摘要: |
| Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult
patients at initial diagnosis of B-cell acute lymphoblastic leukemia (B-ALL).
Methods Relative mRNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells
(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain
reaction (qRT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH
was used as a reference gene.
Results Relative expression of BIM, BCL-6, and c-MYC mRNA in B-ALL patients was significantly lower
than in healthy controls (P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL
patients and those incomplete remission (CR) (P < 0.05). There were no statistically significant differences
in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups (P > 0.05).
Spearman’s rank correlation analyses revealed the expression level of BIM to be positively correlated with
that of BCL-6 in B-ALL patients.
Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients.
Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and
following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for
the diagnosis of adult B-ALL. |
|
View Full Text
Download reader HTML全文 |
| Close |
|
|
|