| Chanli Zheng,Ling Xu,Yanjun Xie,Dongmei He,Yangqiu Li. Expression pattern of BIM, BCL-6, and c-MYC in adult B-cell acute lymphoblastic leukemia. Oncol Transl Med, 2017, 3: 151-155. |
| Expression pattern of BIM, BCL-6, and c-MYC in adult B-cell acute lymphoblastic leukemia |
| Received:November 16, 2016 Revised:July 25, 2017 |
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| KeyWord:BIM; BCL-6; c-MYC; B cell; acute lymphoblastic leukemia (ALL); quantitative reversetranscription polymerase chain reaction (qRT-PCR) |
| Author Name | Affiliation | E-mail | | Chanli Zheng | Jinan University | xiaoliergirl@126.com | | Ling Xu | Jinan University | | | Yanjun Xie | Panyu District Zhongcun Hospital | | | Dongmei He | Jinan University | thedm@jnu.edu.cn | | Yangqiu Li | Jinan University | |
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| Abstract: |
| Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult
patients at initial diagnosis of B-cell acute lymphoblastic leukemia (B-ALL).
Methods Relative mRNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells
(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain
reaction (qRT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH
was used as a reference gene.
Results Relative expression of BIM, BCL-6, and c-MYC mRNA in B-ALL patients was significantly lower
than in healthy controls (P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL
patients and those incomplete remission (CR) (P < 0.05). There were no statistically significant differences
in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups (P > 0.05).
Spearman’s rank correlation analyses revealed the expression level of BIM to be positively correlated with
that of BCL-6 in B-ALL patients.
Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients.
Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and
following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for
the diagnosis of adult B-ALL. |
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