| Guozhao Meng,Junlei Yi,Ming Xie. Differential expression of nucleolin in colon adenoma and adenocarcinoma. Oncol Transl Med, 2017, 3: 82-85. |
| 核仁素在结肠腺瘤和腺癌组织中表达的差异 |
| Differential expression of nucleolin in colon adenoma and adenocarcinoma |
| Received:October 09, 2016 Revised:April 07, 2017 |
| DOI：10.1007/s10330-016-0197-7 |
| 中文关键词: 核仁素；结肠腺瘤；结肠腺癌 |
| 英文关键词: nucleolin; colon adenoma; colon adenocarcinoma |
| 基金项目: |
| Author Name | Affiliation | Department | | Guozhao Meng | School of Fundamental Medicine and Affiliated Hospital, Xiangnan University | Department of Pathology | | Junlei Yi | School of Fundamental Medicine and Affiliated Hospital, Xiangnan University | Department of Pathology | | Ming Xie* | School of Fundamental Medicine and Affiliated Hospital, Xiangnan University | Department of Pathology |
|
| Hits: 10182 |
| Download times: 11380 |
| 中文摘要: |
| 目的 比较核仁素在结肠腺瘤和腺癌组织中表达的差异，探讨核仁素表达在结肠腺癌发生中所起的作用及核仁素表达量与结肠腺癌病理分级的关系。方法 用免疫组化检测80例结肠腺癌及其周围正常肠粘膜组织和60例结肠腺瘤组织中核仁素的表达，比较核仁素在三种组织中表达的差异，并分析核仁素表达量与结肠腺癌病理分级的关系。结果 核仁素在三种组织中均定位表达于细胞核，并表现出不同的表达强度。Allred免疫组化半定量评分显示，核仁素表达量在结肠腺癌组织（7.8±0.1）显著高于结肠腺瘤（6.3±0.2）和正常肠粘膜腺体（5.4±0.1），P＜0.01；在结肠腺瘤显著高于正常肠粘膜腺体，P＜0.01。核仁素表达量在中-高分化结肠腺癌（6.8±0.2）显著低于低分化腺癌（8.0±0.1），P＜0.01。结论 核仁素过度表达在结肠癌的发生和发展中起重要作用。 |
| 英文摘要: |
| Objective The aim of the study was to investigate the discrepancy in nucleolin expression between
colon adenoma and colon adenocarcinoma, explore the role of nucleolin expression in the carcinogenesis
of colon adenocarcinoma, and determine the correlation of the nucleolin expression level with histological
grade in colon adenocarcinoma.
Methods In total, 80 cases of colon adenocarcinoma with cancer-adjacent colon mucosa and 60 cases of
colon adenomas were examined by immunohistochemistry using an antibody against nucleolin. Nucleolin
expression levels in these groups were compared. The correlation between the nucleolin expression level
and grade of colon adenocarcinoma was analyzed.
Results Nucleolin expression is located in the nuclei of colon adenocarcinoma, colon adenoma, and
cancer-adjacent colon mucosa tissues with different intensities. A semiquantitative evaluation using the
Allred scoring system showed that the nucleolin immunostaining score in colon adenocarcinoma (7.8 ± 0.1)
was significantly higher than those in colon adenoma (6.3 ± 0.2) and cancer-adjacent colon mucosa (5.4 ±
0.1; P < 0.01). The nucleolin immunostaining score in colon adenoma was significantly higher than that in
cancer-adjacent colon mucosa (P < 0.01). Nucleolin expression levels in well-differentiated and moderately
differentiated adenocarcinoma (6.8 ± 0.2) were significantly lower than those in poorly differentiated
adenocarcinoma (8.0 ± 0.1; P < 0.01).
Conclusion Increased nucleolin expression may play an important role in the process of malignant
transformation of colon adenocarcinoma and predicts a poor prognosis. |
|
View Full Text
Download reader HTML全文 |
| Close |