| Sumei Chen,Ke Zhang,Yuanyuan Chen,Ruzhen Zheng,Penjun Zhao,Jianwei Zhu,Shuming Wu,Qinghua Deng,Shenglin Ma,Guangsu Xiong. Anticancer effect and enhanced chemotherapy potential of resveratrol in human pancreatic cancer cell lines. Oncol Transl Med, 2016, 2: 156-164. |
| Anticancer effect and enhanced chemotherapy potential of resveratrol in human pancreatic cancer cell lines |
| Received:May 28, 2016 Revised:August 22, 2016 |
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| KeyWord:resveratrol; gemcitabine; pancreatic cancer; apoptosis; proliferation |
| Author Name | Affiliation | E-mail | | Sumei Chen | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Ke Zhang | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Yuanyuan Chen | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Ruzhen Zheng | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Penjun Zhao | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Jianwei Zhu | Department of Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Shuming Wu | Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, China | chensusu1986@126.com | | Qinghua Deng | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | dengqinghua69@163.com | | Shenglin Ma | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | mashenglin@medmail.com.cn | | Guangsu Xiong | Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, China | wusm2002@hotmail.com |
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| Abstract: |
| Objective: Gemcitabine, the only approved drug for the treatment of pancreatic cancer, is not very effective.
Novel and effective cancer chemopreventive agents are urgently needed. Recently, emerging studies
determined resveratrol possessed anticancer effects on various cancer cells. We explored the anticancer
effect of resveratrol in pancreatic cancer cells and investigated the involved moleculars of action. We also
examined whether resveratrol enhanced antitumor activity of gemcitabine in vitro.
Methods: Proliferation inhibition was assessed by cell count kit-8 assay. Cell cycle phase distribution
and apoptotic cells were measured by flow cytometric analysis. We determined the expression of bcl-2,
cyclinD1, and activation of caspases-3 and poly (ADP-ribose) polymerase1 proteins used Western blot
analysis.
Results: Resveratrol inhibited the proliferation of three pancreatic cancer cell lines in a dose dependent
fashion, and induced accumulation of cells at the G1 phase as well as apoptosis. Our data also demonstrated
that resveratrol enhanced gemcitabine-induced apoptosis in pancreatic cancer cells. In addition,
resveratrol inhibited the expression of cyclinD1, bcl-2, and induced activation of caspase-3 and poly (ADPribose)
polymerase1.
Conclusion: Our results suggested that resveratrol might be not only a potential regimen, but also an
effective chemosensitizer for the chemotherapy of pancreatic cancer. |
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