| Sumei Chen,Ke Zhang,Yuanyuan Chen,Ruzhen Zheng,Penjun Zhao,Jianwei Zhu,Shuming Wu,Qinghua Deng,Shenglin Ma,Guangsu Xiong. Anticancer effect and enhanced chemotherapy potential of resveratrol in human pancreatic cancer cell lines. Oncol Transl Med, 2016, 2: 156-164. |
| 白藜芦醇在胰腺癌细胞中的抗肿瘤及化疗增敏作用的研究 |
| Anticancer effect and enhanced chemotherapy potential of resveratrol in human pancreatic cancer cell lines |
| Received:May 28, 2016 Revised:August 22, 2016 |
| DOI：10.1007/s10330-016-0163-7 |
| 中文关键词: 白藜芦醇；吉西他滨；胰腺癌；凋亡；增殖 |
| 英文关键词: resveratrol; gemcitabine; pancreatic cancer; apoptosis; proliferation |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Sumei Chen | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Ke Zhang | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Yuanyuan Chen | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Ruzhen Zheng | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Penjun Zhao | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Jianwei Zhu | Department of Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | chensusu1986@126.com | | Shuming Wu | Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, China | chensusu1986@126.com | | Qinghua Deng | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | dengqinghua69@163.com | | Shenglin Ma | Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou 310002, China | mashenglin@medmail.com.cn | | Guangsu Xiong* | Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, China | wusm2002@hotmail.com |
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| 中文摘要: |
| 目的：吉西他滨是唯一一个在胰腺癌治疗中疗效确切的药物，但效果欠佳。新型抗肿瘤药物十分紧缺。最近，越来越多的研究证明：白藜芦醇在多种肿瘤中有治疗作用。本研究拟对白藜芦醇在胰腺癌中的抗肿瘤作用及分子机制进行研究，另外还对其增强吉西他滨的化疗疗效进行了研究。
方法：CCK-8检测增殖作用，FCM检测周期及凋亡，western blot检测相关蛋白表达情况。
结果：白藜芦醇具有抑制胰腺癌细胞的增殖，引起细胞阻滞在G1期，诱导凋亡的作用。我们还发现白藜芦醇能够增强吉西他滨的化疗疗效，这些作用可能与下调CyclinD1、Bcl-2的表达，及caspase-3、PARP的表达相关。
结论：白藜芦醇可能不仅具有抗肿瘤作用，而且具有增强化疗敏感性的潜能。 |
| 英文摘要: |
| Objective: Gemcitabine, the only approved drug for the treatment of pancreatic cancer, is not very effective.
Novel and effective cancer chemopreventive agents are urgently needed. Recently, emerging studies
determined resveratrol possessed anticancer effects on various cancer cells. We explored the anticancer
effect of resveratrol in pancreatic cancer cells and investigated the involved moleculars of action. We also
examined whether resveratrol enhanced antitumor activity of gemcitabine in vitro.
Methods: Proliferation inhibition was assessed by cell count kit-8 assay. Cell cycle phase distribution
and apoptotic cells were measured by flow cytometric analysis. We determined the expression of bcl-2,
cyclinD1, and activation of caspases-3 and poly (ADP-ribose) polymerase1 proteins used Western blot
analysis.
Results: Resveratrol inhibited the proliferation of three pancreatic cancer cell lines in a dose dependent
fashion, and induced accumulation of cells at the G1 phase as well as apoptosis. Our data also demonstrated
that resveratrol enhanced gemcitabine-induced apoptosis in pancreatic cancer cells. In addition,
resveratrol inhibited the expression of cyclinD1, bcl-2, and induced activation of caspase-3 and poly (ADPribose)
polymerase1.
Conclusion: Our results suggested that resveratrol might be not only a potential regimen, but also an
effective chemosensitizer for the chemotherapy of pancreatic cancer. |
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