| Xiangmin Jia,Hongsheng Yu#. Modulation of MMP-2 and TIMP-2 by low dose radiation in mice bearing S180 sarcoma. Oncol Transl Med, 2016, 2: 127-131. |
| 低剂量照射对S180肉瘤小鼠组织中MMP-2，TIMP-2的影响 |
| Modulation of MMP-2 and TIMP-2 by low dose radiation in mice bearing S180 sarcoma |
| Received:March 30, 2016 Revised:June 08, 2016 |
| DOI：10.1007/s10330-016-0156-6 |
| 中文关键词: 低剂量照射 ； MMP-2； TIMP-2 |
| 英文关键词: MMP-2; TIMP-2; low dose radiation; S180 sarcoma |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Xiangmin Jia | #Department of Oncology,The Affiliated Hospital of Qingdao University | 386303816@qq.com | | Hongsheng Yu#* | #Department of Oncology,The Affiliated Hospital of Qingdao University | qdhsyu@126.com |
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| 中文摘要: |
| 摘要：
目的：本实验旨在探讨低剂量照射对S180小鼠组织中MMP-2和TIMP-2的影响，进一步研究低剂量照射的抗肿瘤效应。
方法：将接种有肿瘤细胞的昆明种S180小鼠随机分成2组，低剂量照射组和对照组。对照组小鼠于接种后12h处死，低剂量照射组分别于12h（LDR12h组），24h（LDR24h组），48h（LDR48h组）和72h（LDR72h组）处死。处死前观察各组小鼠的状态，处死后分别测定肿瘤组织中MMP-2和TIMP-2的表达量。
结果：与对照组相比，低剂量照射组小鼠状态较好；LDR24h组与LDR48h组MMP-2的表达量与对照组相比具有统计学意义；各组TIMP-2的表达差异无统计学意义。
结论：低剂量照射能够通过抑制MMP-2的表达抑制肿瘤的侵袭与转移。 |
| 英文摘要: |
| Objective To investigate the inhibition of low dose radiation (LDR) on S180 sarcomas and its modulation
of MMP-2 and TIMP-2 in mice.
Methods S180 subcutaneously implanted tumor model mice were randomly divided into two groups:
control (N) and low dose radiation (LDR) groups. N mice were sacrificed after 12 h, whereas LDR mice
were sacrificed after 12 (LDR-12 h), 24 (LDR-24 h), 48 (LDR-48 h), and 72 (LDR-72 h) h. Thereafter, we
measured the tumor volumes. Histopathology was performed, and P-V immunohistochemistry was applied
to assess MMP-2 and TIMP-2 expression.
Results Compared with the control group, the tumor growth was significantly inhibited in the LDR groups
(P < 0.05). MMP-2 expression was considerably reduced in LDR-24h (P < 0.05) and LDR-48h (P < 0.05),
whereas the change of TIMP-2 was not obvious in the LDR groups (P > 0.05) in contrast to that of the
control group.
Conclusion LDR can effectively suppress the growth of S180 implanted tumors by reducing MMP-2,
which is associated with invasion and metastasis. |
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