| Xuli Yang,Li Wang,Wenli Sai,Yin Cai,Juanjuan Gu,Xin Chen,Dengfu Yao. Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer. Oncol Transl Med, 2016, 2: 261-267. |
| 肺癌患者癌组织及血缺氧诱导因子-1α表达分析的临床价值 |
| Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer |
| Received:January 16, 2016 Revised:December 02, 2016 |
| DOI：10.1007/s10330-016-0131-1 |
| 中文关键词: ]肺癌；缺氧诱导因子-1α；核酸代谢；酶联免疫吸附测定；诊断 |
| 英文关键词: lung cancer; hypoxia-inducible factor-1α (HIF-1α); nucleic acid metabolism; enzyme-linked immunosorbent assay (ELISA); diagnosis |
| 基金项目:江苏省“六大人才”项目（WSW-011）；南通市应用研究科技计划项目（BK2013048）。 |
| Author Name | Affiliation | Department | | Xuli Yang | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Li Wang | Medical School of Nantong University, Nantong 226001, China | Center of Medical Informatics | | Wenli Sai | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Yin Cai | The Affiliated Hospital of Nantong University, Nantong 226001, China | Department of Oncology | | Juanjuan Gu | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Xin Chen | Nantong University, Nantong 226001, China | School of Life Science | | Dengfu Yao* | Research Center of Clinical Medicine, Affiliated Hospital of Nantong University
| Research Center of Clinical Medicine |
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| 中文摘要: |
| 目的 检测肺癌患者癌组织及外周血缺氧诱导因子-1α（HIF-1α）表达水平并分析其临床价值。方法 选取2012年1月至2015年2月该院收治的肺癌患者115例作为肺癌组，选取同期住院肺部感染肺炎患者30例作为肺炎组、健康体检者30例作为健康对照组。收集115例肺癌患者术后癌及癌旁组织标本，提取总RNA分析组织核酸代谢水平；采用组织芯片和免疫组织化学法分析HIF-1α表达状态与胞内分布；采用酶联免疫吸附法定量检测外周血HIF-1α及血管内皮生长因子（VEGF）水平。结果 肺癌组患者血清HIF-1α水平[（138.3±28.8）μg/L]明显高于健康对照组[（24.1±3.3）μg/L]和肺炎组[（58.8±14.5）μg/L]，差异均有统计学意义（P<0.01），且与血清VEGF水平呈显著正相关（r=0.937， P<0.01）；肺癌总RNA比浓度[（1.52±1.14 μg/mg）]显著高于癌周组织[（0.58± 0.33 μg/mg）] ，差异有统计学意义（t=8.494， P<0.01）；不同性别、肿瘤直径、分化程度及有无淋巴结转移患者肺癌组织HIF-1α表达阳性率比较，差异均有统计学意义（P<0.05）。结论 肺癌组织HIF-1α表达与组织核酸代谢水平和血管形成密切相关，且有助于肺癌的诊断与鉴别。 |
| 英文摘要: |
| Objective The aim of the study was to investigate the clinicopathological characteristics of hypoxiainducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression in patients with lung
cancer.
Methods Cancerous and noncancerous tissues were collected post-operation from 115 patients with lung
cancers by the self-control method. Total RNA was extracted from the lung tissues. The status of tissue HIF-
1α expression and intercellular distribution was observed by immunochemistry using a tissue microarray.
The expression levels of circulating HIF-1α and VEGF were detected by enzyme-linked immunosorbent
assay (ELISA).
Results The expression of serum HIF-1α [(138.3 ± 28.8) μg/L] in the group of patients with lung cancer
was significantly higher (P < 0.01) than that in the group of patients with pneumonia [(58.8 ± 14.5) μg/L] and
the control group of patients [(24.1 ± 3.3) μg/L]. There was a strong positive correlation of serum HIF-1α
levels (r = 0.937, P < 0.01) with serum VEGF levels. The specific concentration of total RNA [(1.52 ± 1.14)
μg/mg wet lung tissues] in the cancerous tissues was significantly higher (t = 8.494, P < 0.001) than that in
the noncancerous tissues [(0.58 ± 0.33) μg/mg]. The clinicopathological features of HIF-1α expression in
lung cancer tissues revealed a significant relationship between positive HIF-1α expression and patient sex
(χ2 = 4.494, P = 0.034), tumor size (χ2 = 4.679, P = 0.031), differentiation degree (χ2= 8.846, P = 0.012),
and presence of lymphatic node metastasis (χ2= 6.604, P = 0.037).
Conclusion Abnormal HIF-1α expression in lung cancer is closely related with nucleic acid metabolism
and angiogenesis, and it may be helpful in the diagnosis and identification of lung cancer. |
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