| Qing Dong,Hongsheng Yu. Low-dose radiation reverses cisplatin resistance in ovarian cancer cells by changing Survivin and Caspase-3 expression. Oncol Transl Med, 2016, 2: 90-95. |
| 低剂量辐射通过Survivin和Caspase-3逆转卵巢癌细胞的顺铂耐药 |
| Low-dose radiation reverses cisplatin resistance in ovarian cancer cells by changing Survivin and Caspase-3 expression |
| Received:November 05, 2015 Revised:April 07, 2016 |
| DOI：10.1007/s10330-015-0122-8 |
| 中文关键词: 关键词：SKOV3/DDP,低剂量辐射，Survivin,Caspase-3, |
| 英文关键词: SKOV3/DDP; LDR; Caspase-3; Survivin |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Qing Dong | The Affiliated Hospital of Qingdao University | dong_1063397@126.com | | Hongsheng Yu* | The Affiliated Hospital of Qingdao University | qdhsyu@126.com |
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| 中文摘要: |
| 摘要：目的：顺铂是卵巢癌的主要化疗药物。但是在临床应用中卵巢癌细胞往往会出现对顺铂的耐药。肿瘤细胞对低剂量辐射敏感。低剂量辐射治疗可以提高卵巢癌对化疗药物的敏感性，但是机制却不是很清楚。本课题将研究低剂量辐射通过对Survivin和Caspase-3两种基因的影响来逆转卵巢癌细胞对顺铂的耐药的机制。方法：应用CCK8法检测低剂量辐射对SKOV3/DDP细胞活性的影响，应用流式细胞术检测肿瘤细胞凋亡，real-time PCR技术检测肿瘤细胞耐药相关蛋白Caspase-3和Survivin的表达。结果：低剂量辐射组的细胞增殖活性比对照组和常规照射组的低（P<0.05）。低剂量辐射组，对照组和常规照射组的IC50分别是：3.837±0.16,9.467±0.17和9.389±0.17。低剂量辐射组的Caspase-3 mRNA升高，Survivin mRNA降低（P<0.05）。结论：低剂量辐射可能通过抑制Survivin的表达水平，提高Caspase-3的表达水平，促进SKOV3/DDP细胞的凋亡来逆转卵巢癌细胞的耐药。 |
| 英文摘要: |
| Objective Cisplatin (DDP) is the main chemotherapy drug for ovarian cancer. However, ovarian cancer cells tend to develop cisplatin resistance in the clinical setting. Tumor cells are sensitive to low-dose radiation (LDR). LDR therapy can improve the effects of chemotherapy drugs on ovarian cancer, but the underlying mechanisms are not clear. In this study, we explored the impact of low-dose radiation on Survivin and Caspase-3 levels in SKOV3/DDP ovarian cancer cells that are resistant to cisplatin. Methods Cell viability was examined by cell counting kit-8 (CCK-8) assay, and quantitative PCR was used to detect Caspase-3 and Survivin transcript levels. Flow cytometry was used to detect and quantify apoptotic cells.Results Cell viability was lower when cells were treated with LDR and cisplatin than when cells were treated with conventional radiation and cisplatin, or cisplatin alone (P < 0.05). The IC50 of cisplatin in the LDR, no-radiation control, and conventional-dose groups was 3.837 ± 0.16, 9.467 ± 0.17, and 9.389 ±0.17, respectively. The level of Caspase-3 mRNA was higher and the level of Survivin mRNA was lower in the LDR group compared to that in the other two groups (P < 0.05). Conclusion LDR reverses cisplatin resistance in SKOV3/DDP cells, and may do so by suppressing Survivin expression and increasing Caspase-3 expression. |
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