| You Zou,Xiaolan Li,Deding Tao,Junbo HU,Jianping Gong. Epidermal growth factor enhances chemosensitivity of colon cancer by inducing cancer stem cells to enter the cell cycle. Oncol Transl Med, 2017, 3: 86-90. |
| Epidermal growth factor enhances chemosensitivity of colon cancer by inducing cancer stem cells to enter the cell cycle |
| Received:April 30, 2015 Revised:April 03, 2017 |
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| KeyWord:chemosensitivity; tumor stem cell; cell cycle |
| Author Name | Affiliation | E-mail | | You Zou | Department of General Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology | melonlan@163.com | | Xiaolan Li | Department of General Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology | yzou2003@163.com | | Deding Tao | Department of General Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology | 63678351@qq.com | | Junbo HU | Department of General Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology | 674307118@qq.com | | Jianping Gong | Department of General Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology | 674307119@qq.com |
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| Abstract: |
| Objective The aim of the study was to investigate whether colon cancer stem cells induced by epidermal
growth factor (EGF) to enter the cell cycle enhanced the chemosensitivity of colon cancer.
Methods In vitro, EGF was used to stimulate the entry of human colon cancer HCT116 cells into the cell
cycle. Before and after treatment with EGF, CD133 HCT116 cells were collected and flow cytometry was
conducted to determine the apoptosis rate based on the 5-Fu and Ki-67 expression rates. The cell cycle
distribution of the two groups was also determined. In vivo, a subcutaneous xenograft model of HCT116
human colon cancer cell lines in nude mice was established. The nude mice were divided into two groups
and treated with EGF and 5-Fu, respectively. Differences in the growth of implanted tumors revealed the
efficiency of cycle-induction combined chemotherapy.
Results (1) After EGF stimulation, the S-G2/M proportion of CD133 HCT116 cells and Ki67 expression
were increased, indicating that more cancer stem cells entered the cell cycle and promoted proliferation; (2)
After EGF stimulation, CD133 HCT116 cells showed a higher apoptosis rate induced by 5-Fu. (3) Animal
experiments showed that the group subjected to combined treatment with EGF and 5-Fu had smaller tumor
sizes compared to the group treated with 5-Fu alone.
Conclusion EGF enhanced tumor sensitivity to chemotherapeutic drugs, likely by promoting tumor stem
cells to enter the cell cycle. |
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