| Xuebin Ma,Cong Ma,Wei Qiu,Hongxia Yuan,Ping Yang,Jinbo Kang. In vitro incubation of cytokine-induced killer cells from patients with and without hepatitis B virus and a cell subset analysis. Oncol Transl Med, 2015, 1: 275-279. |
| 乙型肝炎病毒表面抗原对实体肿瘤患者CIK细胞诱导培养及表型分析的影响 |
| In vitro incubation of cytokine-induced killer cells from patients with and without hepatitis B virus and a cell subset analysis |
| Received:March 16, 2015 Revised:November 21, 2015 |
| DOI：10.1007/s10330-015-0078-8 |
| 中文关键词: 乙型肝炎表面抗原，细胞因子诱导的杀伤细胞，细胞亚群 |
| 英文关键词: hepatitis B virus (HBV); cytokine-induced killer cells (CIKs); immune cell subset |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Xuebin Ma* | Tumor diagnosis and treatment center of Navy General Hospital | 1965394316@qq.com | | Cong Ma | Tumor diagnosis and treatment center of Navy General Hospital | macong66858166@163.com | | Wei Qiu | Tumor diagnosis and treatment center of Navy General Hospital | 2670738391@qq.com | | Hongxia Yuan | Tumor diagnosis and treatment center of Navy General Hospital | 37296862@qq.com | | Ping Yang | Tumor diagnosis and treatment center of Navy General Hospital | yangping177@aliyun.com | | Jinbo Kang | Tumor diagnosis and treatment center of Navy General Hospital | kjbnet@126.com |
|
| Hits: 8519 |
| Download times: 10910 |
| 中文摘要: |
| 目的：探讨乙型肝炎表面抗原阳性患者与阴性患者细胞因子诱导的杀伤细胞（CIK）体外培养过程中个细胞增殖情况和淋巴细胞亚群的变化。方法：分别收集本院住院肿瘤患者50例，根据乙肝表面抗原表达情况分为阴性组和阳性组。分别抽取患者外周血进行CIK细胞诱导培养，在第1、5、7、9、11、13、15天进行细胞活力检测并计数，第5、7、10、13、15天进行各细胞亚群的表达分析。结果：两组患者CIK细胞增殖情况单个时间点比较无显著性差异，但是在第15天的增殖倍数阳性组显著高于阴性组（280倍＆180倍）；细胞亚群分析中CD3+T细胞、CD8 T细胞、CD3 CD8 T细胞、CD3 CD56 T细胞比例均随培养时间延长而升高，CD4 T细胞、CD3 CD4 T细胞随培养时间延长而降低，阳性组更显著；其中CD8 T细胞和CD3 CD4 T细胞在两组间的差异具有统计学意义（p<0.05）。结论：乙型肝炎表面抗原阳性患者CIK细胞较阴性患者扩增能力更强，效应细胞表型表达更有利于杀伤作用的发挥。 |
| 英文摘要: |
| Objective?The aim of the study was to explore the difference between immune cell subsets during the incubation of cytokine-induced kill cells (CIKs) from patients with and without hepatitis B virus (HBV). Methods?Peripheral blood samples were extracted from 50 tumor patients, and were divided into two groups according to the presence or absence of HBV. The proliferation rate and activity of CIK cells were examined based on counts on days 1, 5, 7, 9, 11, 13, and 15 of culture. Additionally, the CD3 , CD4 , CD8 , CD3 CD8 , CD3 CD4 , and CD3 CD56 T cell populations were analyzed by flow cytometry on days 5, 7, 10, 13, and 15 of culture.
Results?Proliferation over a 15-day period was higher in the HBV-positive group than in the negative group (280-fold vs. 180-fold increase, respectively), but there was no significant difference between the two groups at each time point. The frequencies of CD3 , CD8 T, CD3 CD8 , and CD3 CD56 T cells increased over time, while those of CD4 and CD3 CD4 T cells decreased over time, and these changes were greater in the positive group than in the negative group. The differences in CD8 T cells and CD3 CD4 T cells between the two groups were significant (P < 0.05).
Conclusion?The proliferative capacity of CIK cells was higher for patients in the HBV-positive group than those in the HBV-negative group, and immune cell subsets were more favorable in the HBV-positive group than the negative group.
|
|
View Full Text
Download reader HTML全文 |
| Close |
|
|
|