| Mingxuan Chen,Yongheng An,Hongsheng Yu,Yifan Li,Xiaoman Chen. Damage of Wistar rats liver after using hypo-fractionated radiation and oxaliplation. Oncol Transl Med, 2015, 1: 130-134. |
| Damage of Wistar rats liver after using hypo-fractionated radiation and oxaliplation |
| Received:February 08, 2015 Revised:June 02, 2015 |
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| KeyWord:radiation effects; concurrent chemoradiotherapy; rat liver; Bcl-2; Bax |
| Author Name | Affiliation | E-mail | | Mingxuan Chen | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | chenmingxuancc@sina.com | | Yongheng An | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | anyongheng@126.com | | Hongsheng Yu | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | qdhsyu@163.com | | Yifan Li | Department of Breast Center,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | 21729860@163.com | | Xiaoman Chen | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | 465146825@qq.com |
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| Abstract: |
| Objective The purpose of this study was to clarify whether hypo-fractionated radiation therapy combined
with oxaliplatin can aggravate liver damage, in order to determine its safety for clinical application.
Methods Eighty Wistar rats were randomly divided into four groups: the control group, the chemotherapy
treatment group, the radiation treatment group, and the concurrent chemoradiotherapy group. The rats’ liver
tissues were then collected for histological evaluation at the first, second, fourth, sixth, and eight week after
irradiation. The tissues were histologically evaluated using hematoxylin and eosin staining, and immunohistochemistry to analyze the expression of Bcl-2 and Bax.
Results Histological examination revealed swollen hepatocellular cells in the experimental groups, with
visible liver degeneration and necrosis. Alanine aminotransferase and aspartate aminotransferase levels
were significantly different between the groups (F = 85.869 and 214.663; P < 0.001). The intra-group expressions of Bcl-2 and Bax were also significantly different between each time point (F = 6.047 and 43.344;
P < 0.05). Bax expression was significantly different between each group (F = 8.122; P < 0.05), although no
inter-group differences were observed for Bcl-2 expression (F = 0.808; P > 0.05).
Conclusion Chemoradiotherapy may aggravate liver injury, possible via overexpression of Bcl-2 and
reduced expression of Bax. Therefore, this treatment should be used carefully in the clinic. |
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