| Mingxuan Chen,Yongheng An,Hongsheng Yu,Yifan Li,Xiaoman Chen. Damage of Wistar rats liver after using hypo-fractionated radiation and oxaliplation. Oncol Transl Med, 2015, 1: 130-134. |
| 低分割X线照射同期联合奥沙利铂 对大鼠肝脏的损伤性研究 |
| Damage of Wistar rats liver after using hypo-fractionated radiation and oxaliplation |
| Received:February 08, 2015 Revised:June 02, 2015 |
| DOI：10.1007/s10330-015-0070-3 |
| 中文关键词: 放射生物学效应；同期放化疗；肝脏；bcl-2；bax |
| 英文关键词: radiation effects; concurrent chemoradiotherapy; rat liver; Bcl-2; Bax |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Mingxuan Chen | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | chenmingxuancc@sina.com | | Yongheng An* | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | anyongheng@126.com | | Hongsheng Yu | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | qdhsyu@163.com | | Yifan Li | Department of Breast Center,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | 21729860@163.com | | Xiaoman Chen | Department of Oncology,The Affiliated Hospital of Qingdao University,Qingdao,Shandong | 465146825@qq.com |
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| 中文摘要: |
| 目的：探讨低分割X线照射同期联合奥沙利铂对大鼠肝脏损伤的影响，为其临床应用提供参考。方法：选择Wistar大鼠80只，随机分为对照组（A组）、化疗组（B组）、放疗组（C组）和同步放化疗组（D组）。分别在第1、2、4、6、8周后解剖大鼠肝脏，通过HE染色观察肝脏形态学变化，测定谷氨酰丙转氨酶(ALT)，谷氨酰转氨酶（AST）评估肝功能，并应用免疫组织化学方法检测Bcl-2和Bax蛋白的表达。结果：实验组肝细胞均有不同程度的肿胀、变性、坏死，各组间的ALT、AST在同一时间点有统计学差异（F = 85.869、214.663, P < 0.001）。Bcl-2和Bax蛋白的表达在实验组各时间点有统计学差异（F =6.047, 43.344 P < 0.05）,Bcl-2在同一时间点不同组间无明显统计学差异（F=0.808 P＞0.05）。 结论：与化疗组和放疗组相比，同步放化组可能会导致大鼠肝脏损伤加剧，其机制可能与抗凋亡基因Bcl-2表达减少,凋亡基因Bax表达增多有关，在临床应用中应注意对肝脏的保护。 |
| 英文摘要: |
| Objective The purpose of this study was to clarify whether hypo-fractionated radiation therapy combined
with oxaliplatin can aggravate liver damage, in order to determine its safety for clinical application.
Methods Eighty Wistar rats were randomly divided into four groups: the control group, the chemotherapy
treatment group, the radiation treatment group, and the concurrent chemoradiotherapy group. The rats’ liver
tissues were then collected for histological evaluation at the first, second, fourth, sixth, and eight week after
irradiation. The tissues were histologically evaluated using hematoxylin and eosin staining, and immunohistochemistry to analyze the expression of Bcl-2 and Bax.
Results Histological examination revealed swollen hepatocellular cells in the experimental groups, with
visible liver degeneration and necrosis. Alanine aminotransferase and aspartate aminotransferase levels
were significantly different between the groups (F = 85.869 and 214.663; P < 0.001). The intra-group expressions of Bcl-2 and Bax were also significantly different between each time point (F = 6.047 and 43.344;
P < 0.05). Bax expression was significantly different between each group (F = 8.122; P < 0.05), although no
inter-group differences were observed for Bcl-2 expression (F = 0.808; P > 0.05).
Conclusion Chemoradiotherapy may aggravate liver injury, possible via overexpression of Bcl-2 and
reduced expression of Bax. Therefore, this treatment should be used carefully in the clinic. |
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