| Long Qin,Jing Ge. Expression of PinX1 and hTERT in basal cell carcinoma and their implications. Oncol Transl Med, 2015, 1: 140-143. |
| Pinx l和hTERT在基底细胞癌组织中的表达及意义 |
| Expression of PinX1 and hTERT in basal cell carcinoma and their implications |
| Received:January 24, 2015 Revised:June 02, 2015 |
| DOI：10.1007/s10330-015-0065-0 |
| 中文关键词: Pinxl，hTERT，基底细胞癌，实时定量PCR |
| 英文关键词: telomerase inhibitor 1 (PinX1); human telomerase reverse transcriptase (hTERT); basal cell carcinoma; real-time polymerase chain reaction; immunohistochemistry |
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| 中文摘要: |
| 摘要：
目的探讨内源性端粒酶抑制基因PinX1和端粒酶逆转录酶hTERT在基底细胞癌组织中的表达及其意义。方法采用实时定量PCR方法检测30例基底细胞癌组织中内源性端粒酶抑制基因PinXl和端粒酶逆转录酶hTERT的mRNA表达水平，并设15例正常人皮肤组织作为对照组。结果实验组PinXl基因的mRNA表达水平(2．75±1．07)低于对照组(4.33±2．92)，两者差异具有统计学意义(t=4．128，P<0．05)；hTERT基因的mRNA表达水平(5．64±2．37)明显高于对照组(1．55±0．58)，两者差异具有统计学意义(t=5．413，P<0．05)；Pinxl的表达水平与hTERT表达的相关性无统计学意义(P>0．05 )。结论：PinXl的下调及hTERT上调可能与基底细胞癌发生过程中端粒酶激活及维持机制有关。 |
| 英文摘要: |
| Objective This study aimed to investigate the expression and significance of PIN2/TERF1 interacting,
telomerase inhibitor 1 (PinX1) and human telomerase reverse transcriptase (hTERT) in basal cell carcinoma (BCC).
Methods Real-time polymerase chain reaction and immunohistochemistry were performed to quantify
the mRNA expressions and integrated optical density (IOD), respectively, of PinX1 and hTERT in BCC
specimens (n = 30), as well as in normal skin specimens (n = 15).
Results The mRNA expression level and IOD of PinX1 in the BCC samples were both significantly lower
than those in the control specimens (P < 0.05). Conversely, the mRNA expression level and IOD of hTERT
in BCC were both significantly higher than that in the control samples (P < 0.05). The correlation between
the expression levels of PinX1 and hTERT showed no statistical significance (P > 0.05).
Conclusion Downregulation of PinX1 and upregulation of hTERT expression may be associated with the
activation and maintenance of telomerases in the induction of BCC. |
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