| Genhai Zhu,Lan Hong,Shengtan Wang,Zhaoxin Yang,Chunying Chen,Shixuan Wang. Expression of Bmi-1 and EZH2 in tissues adjacent to human epithelial ovarian cancer cells of orthotopic implantation in nude mice. Oncol Transl Med, 2015, 1: 42-47. |
| 人卵巢上皮癌裸鼠原位移植瘤癌旁组织Bmi-1和 EZH2的表达 |
| Expression of Bmi-1 and EZH2 in tissues adjacent to human epithelial ovarian cancer cells of orthotopic implantation in nude mice |
| Received:January 21, 2015 Revised:January 29, 2015 |
| DOI：10.1007/s10330-015-0063-2 |
| 中文关键词: 卵巢上皮癌; 肿瘤相关因子; 基因表达; 癌旁组织 |
| 英文关键词: epithelial ovarian cancer; tumor-associated gene; gene expression; paraneoplastic tissue |
| 基金项目:海南省卫生厅科研课题（琼卫2013资助-040号） |
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| 中文摘要: |
| 目的：通过检测人卵巢上皮癌裸鼠原位移植瘤癌旁组织的Bmi-1 and EZH2表达，探讨筛选癌旁残余正常卵巢组织的可行性。
方法：人卵巢上皮癌OVCAR3细胞皮下种植获取瘤源并卵巢原位移植后，取癌组织、癌旁近端组织及、癌旁远端组织及正常裸鼠卵巢组织，采用RT-PCR及免疫组化法检测以上组织的Bmi-1 and EZH2的表达量。
结果：40例人卵巢上皮癌裸鼠原位移植瘤模型中获取35份活检正常的癌旁残余卵巢组织，获取率87.5%（35/40）。癌旁近端组织中Bmi-1 and EZH2的表达水平低于癌组织（P ＜0.05）,高于癌旁远端组织( P ＜0.01)；不同病变程度获取的残余正常卵巢组织中的表达量差异无统计学意义(P ＞ 0.05)。20例正常裸鼠卵巢组织Bmi-1 and EZH2的表达均为阴性。
结论：Bmi-1 and EZH2等分子指标的表达量向无癌方向呈一递减趋势，这些肿瘤相关基因表达阴性可作为筛选癌旁残余正常卵巢组织的标准，卵巢上皮癌旁可以获取相对安全的正常卵巢组织。 |
| 英文摘要: |
| Objective This study investigated the feasibility of screening residual normal ovarian tissues based on the expression of Bmi-1 and EZH2 in tissues adjacent to orthotopic ovarian carcinomas in nude mice.
Methods The human epithelial ovarian cancer cell line OVCAR3 was grown in subcutaneous tissues and the tumor tissues were orthotopically implanted. The expression levels of Bmi-1 and EZH2 were detected
by immunohistochemical staining and RT-PCR in cancer tissues, proximal and remote tissues with respect to the cancer tissues, and normal ovarian tissues of nude mice.
Results Thirty-five ovarian tissue samples with normal biopsy results were obtained from 40 cases of human epithelial ovarian cancer in the nude mice in which the tumor tissues were orthotopically implanted.
Bmi-1 and EZH2 expression levels were lower in proximal paraneoplastic tissue samples than in cancer tissue samples (P < 0.05) and higher than in remote paraneoplastic tissue samples (P < 0.01). No significant difference was found in the expression levels of Bmi-1 and EZH2 using immunohistochemistry among residual normal ovarian tissues obtained from orthotopically implanted models that differed in severity. The expression of Bmi-1 and EZH2 was negative in 20 normal ovarian tissue samples.
Conclusion The expression levels of Bmi-1 and EZH2 were reduced with increasing distance from the cancer tissues. Negative expression of these tumor-associated genes can be used as a standard for the
screening of normal ovarian tissues adjacent to tumor tissues. Normal ovarian tissues can be obtained from the tissues adjacent to tumors. |
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