| Wei Zhuang,Limin Lun. Preliminary research on dendritic cells loaded with resistant breast cancer antigens in breast cancer-bearing nude mice. Oncol Transl Med, 2015, 1: 120-124. |
| 负载耐受性乳腺肿瘤抗原的DC在乳腺癌裸鼠中的研究初探 |
| Preliminary research on dendritic cells loaded with resistant breast cancer antigens in breast cancer-bearing nude mice |
| Received:January 16, 2015 Revised:February 10, 2015 |
| DOI：10.1007/s10330-015-0062-3 |
| 中文关键词: 树突状细胞； 耐受性；ATP-PCA；乳腺癌；裸鼠 |
| 英文关键词: ATP-TCA; breast cancer; dendritic cell; nude mice; resistance |
| 基金项目:2013年青岛市科技局民生科技计划 |
| Author Name | Affiliation | E-mail | | Wei Zhuang | Medical College of Qingdao University | zw2008006@163.com | | Limin Lun* | The Affiliated Hospital of Qingdao University | lunlmqd@163.com |
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| 中文摘要: |
| 摘要 目的: 探讨负载耐受性乳腺肿瘤抗原的树突状细胞(dendritic cell，DC)对乳腺癌裸鼠的抑瘤效果. 方法: 以原代乳腺肿瘤制备单细胞悬液，使用ATP药敏检测系统筛选化疗药物，并渐次用其1/10IC50、1/5IC50、1/2IC50、1IC50、2IC50值浓度冲击处理肿瘤细胞，直至稳定生长于2IC50值的培养基中。从患者外周血用白细胞单采术分离获得PBMCs，其中贴壁细胞经GM-CSF和IL-4诱导产生DCs，并分别负载耐药株细胞裂解复合物和未经耐药处理的肿瘤细胞裂解复合物，前者获得负载耐受性肿瘤抗原的成熟DCs。以上述耐药株建立乳腺癌荷瘤裸鼠模型，并随机分为三组：治疗组为耐受性肿瘤抗原负载的DC，对照组为原代肿瘤细胞抗原负载的DC，空白组为同体积生理盐水，观察荷瘤变化并绘制生长曲线。结果: 各组小鼠肿瘤体积和重量在治疗前后差异均有统计学意义(P<0.05), 空白组小鼠治疗后肿瘤体积最大(3.362±0.068)cm3，瘤重(637.50±59.398)mg。实验组肿瘤体积最小(1.273±0.071)cm3，瘤重(206.81±32.711)mg. 结论: 负载耐受性乳腺肿瘤抗原的DC对耐药乳腺癌裸鼠模型的抑瘤效果显著，对同种耐药肿瘤的裸鼠抑瘤能力最强。 |
| 英文摘要: |
| Objective The aim of the study was to investigate the inhibitory effects of dendritic cells (DCs) loaded
with resistant breast cancer antigens on breast cancer in nude mice.
Methods A single-cell suspension was prepared from a primary breast cancer and chemotherapeutic
drugs were screened using the ATP-PCA susceptibility testing system. Cancer cells were treated with 1/10
× IC50, 1/5 × IC50, 1/2 × IC50, 1 × IC50, and 2 × IC50 medium until their growth became steady in the 2 × IC50
medium. Peripheral blood mononuclear cells (PBMCs) were obtained from the peripheral blood of patients
with leukapheresis. The obtained adherent cells were induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) to generate DCs, which carried resistant strain cell lysis
compounds or non-treated cancer cell lysis compounds. The former mature DCs carried resistant breast
tumor antigens. A breast tumor-bearing nude mouse model was established with these resistant strains and
the mice were randomly divided in three groups. The mice in the treatment group were injected with DCs
loaded with resistant breast cancer antigens. The control group consisted of mice injected with DCs loaded
with primary tumor cell antigens and the blank group consisted of mice injected with the same volume of
normal saline. Changes in the cancers were observed.
Results After treatment with the effector cells, the cancer volume and weight were significantly different
to those before treatment in every group of mice (P < 0.05). The tumor volume in the blank group was the
largest (3.362 ± 0.068 cm3) and the tumor weight was 637.50 ± 59.398 mg. Compared to the blank group,
the tumor volume in the experimental group was the smallest (1.273 ± 0.071 cm3) and the tumor weight
was 206.81 ± 32.711 mg.
Conclusion DCs loaded with resistant breast cancer antigens demonstrated a significant inhibition effect
on the cancers of breast tumor-bearing nude mice. |
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