| Yan Shi,Yana Li,Naie Li,Min Yu,Dong Wang,Lijun Kong. Ethanolic extract of propolis induces apoptosis of HL-60 cells in vitro. Oncol Transl Med, 2016, 2: 34-38. |
| 蜂胶醇提取物诱导HL-60细胞凋亡的体外实验研究 |
| Ethanolic extract of propolis induces apoptosis of HL-60 cells in vitro |
| Received:December 29, 2014 Revised:October 22, 2015 |
| DOI：10.1007/s10330-014-0047-7 |
| 中文关键词: 蜂胶，白血病，凋亡，Bcl-2/Bax |
| 英文关键词: propolis; leukemia; apoptosis; Bcl-2/Bax |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Yan Shi | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Yana Li | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Naie Li | Department of Diagnostics, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Min Yu | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Dong Wang | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Lijun Kong* | Department of Biochemistry, Binzhou Medical College, Yantai 264003, China | kong_lijun@163.com |
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| 中文摘要: |
| 目的：研究蜂胶醇提取物对HL-60细胞的生长抑制和凋亡诱导作用。方法：用不同蜂胶醇提取物浓度(0μg/ml, 50μg/ml, 100μg/ml and 200μg/ml)体外24h, 48h, 72h分别作用于HL-60细胞，MTT检测法测定细胞增殖抑制率， Hochest33258染色法和TUNEL技术检测细胞凋亡情况，并运用免疫组织化学方法观察蜂胶醇提取物对HL-60细胞Bax和Bcl-2表达的调节。结果：MTT分析显示经不同浓度蜂胶醇提取物对你HL-60细胞增殖有明显影响(P<0.05)。TUNEL检测和Hochest33258染色后荧光显微镜下可以观察到典型的凋亡形态学改变；免疫组化方法显示，蜂胶醇提取物处理组HL-60细胞中Bax表达较对照组明显增加，Bcl-2表达明显降低(P<0.05)，Bcl2/Bax比值增大。结论：蜂胶醇提取物体外能明显抑制HL-60细胞的增殖并诱导其凋亡，其作用的发挥可能与调节Bax和Bcl-2表达，上调Bcl-2/Bax比值有关。 |
| 英文摘要: |
| Objective: The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells.
Methods: HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis (0, 50, 100, and 200 μg/mL). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-iphenyltetrazolium bromide (MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry.
Results: MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation (P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated (P < 0.05).
Conclusion: Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio. |
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