| Jun Bai,Yingxia Ning,Yanfen Chen,Hanzhen He,Wanyu Xie. Induction of apoptosis of human ovarian cancer cells by SGI-1776 combination with DDP in sub-toxic concentration in vitro. Oncol Transl Med, 2014, 13: 589-593. |
| 亚毒性浓度下顺铂联合SGI-1776体外诱导人卵巢癌细胞凋亡 |
| Induction of apoptosis of human ovarian cancer cells by SGI-1776 combination with DDP in sub-toxic concentration in vitro |
| Received:October 28, 2014 Revised:December 12, 2014 |
| DOI：10.1007/s10330-314-0018-4 |
| 中文关键词: 卵巢癌；顺铂；SGI-1776；凋亡 |
| 英文关键词: ovarian cancer; SGI-1776; DDP; apoptosis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Jun Bai | Department of Gynecology and Obstetrics, Maternity and Child Care Hospital of Longgang District of Shenzhen City, Shenzhen 518172, China Hospital of Guangzhou Medical University | shushuanlao@163.com | | Yingxia Ning* | Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China | shushuanlao@163.com | | Yanfen Chen | Department of Gynecology and Obstetrics, Wuhan Women and Children Medical and Healthy Center, Wuhan 430000, China | | | Hanzhen He | Department of Gynecology and Obstetrics, Shenzhen Longgang District Maternity and Child Healthcare Hospital, Shenzhen 518172, China | | | Wanyu Xie | Department of Gynecology and Obstetrics, The First Affiliated Hospital of University of South China, Hengyang 421001, China | |
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| 中文摘要: |
| 目的 探讨顺铂（DDP）联合SGI-1776诱导体外培养人卵巢癌HO-8910细胞凋亡作用。方法 体外培养HO-8910细胞；MTT检测顺铂联合SGI-1776对HO-8910细胞生长增殖的影响；FCM检测细胞凋亡率的影响；Western blot分析蛋白表达变化。结果 DDP和SGI-1776在亚毒性条件下对HO-8910细胞生长有轻微抑制作用，与NS组、DDP组、SGI-1776组比较，DDP联合SGI-1776对人卵巢癌HO-8910细胞生长有明显抑制作用（P﹤0.01），而且其表现为协同作用；经DDP联合SGI-1776作用48 h后，HO-8910细胞出现亚二倍峰，同时bcl-2蛋白表达降低， Bax蛋白Cyto-c蛋白增高。 结论 DDP联合SGI-1776具有诱导体外培养人卵巢癌HO-8910细胞凋亡的作用，而且其表现为协同作用，其机制可能是通过调节线粒体凋亡途径实现的。 |
| 英文摘要: |
| Objective: The aim of the study was to investigate the effect of SGI-1776 combination with DDP in sub-toxic concentration on induction of apoptosis of human ovarian cancer HO-8910 cells in vitro and to unravel the associated mechanisms. Methods: Human ovarian cancer HO-8910 cells were cultured in vitro. The inhibitory effect of SGI-1776 combination with DDP in sub-toxic concentration on induction on viability of human ovarian cancer HO-8910 cells was evaluated by the MTT assay. Cell apoptosis rate was analyzed by flow cytometry. The proteins expression level related to apoptosis were analyzed by Western blot. Results: SGI-1776 combination with DDP in sub-toxic concentration significantly inhibited the proliferation of human ovarian cancer HO-8910 cells, and proliferation inhibition rate was increased drastically compared with normal saline (NS) group or DDP group in sub-toxic concentration or SGI-1776 group in sub-toxic concentration (P﹤0.01). Apoptosis rate markedly increased after the treatment of SGI-1776 combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of Bax and Cyto-c were depressed by SGI-1776 combination with DDP in sub-toxic concentration. Conclusion: SGI-1776 combination with DDP in sub-toxic concentration could inhibit the cell proliferation and lead to cell apoptosis inhuman ovarian cancer HO-8910 cells, and its mechanism may be related to through mitochondrial apoptotic pathway. |
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