| Jun Bai,Yingxia Ning,Yanfen Chen,Hanzhen He,Wanyu Xie. Induction of apoptosis of human ovarian cancer cells by SGI-1776 combination with DDP in sub-toxic concentration in vitro. Oncol Transl Med, 2014, 13: 589-593. |
| Induction of apoptosis of human ovarian cancer cells by SGI-1776 combination with DDP in sub-toxic concentration in vitro |
| Received:October 28, 2014 Revised:December 12, 2014 |
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| KeyWord:ovarian cancer; SGI-1776; DDP; apoptosis |
| Author Name | Affiliation | E-mail | | Jun Bai | Department of Gynecology and Obstetrics, Maternity and Child Care Hospital of Longgang District of Shenzhen City, Shenzhen 518172, China Hospital of Guangzhou Medical University | shushuanlao@163.com | | Yingxia Ning | Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China | shushuanlao@163.com | | Yanfen Chen | Department of Gynecology and Obstetrics, Wuhan Women and Children Medical and Healthy Center, Wuhan 430000, China | | | Hanzhen He | Department of Gynecology and Obstetrics, Shenzhen Longgang District Maternity and Child Healthcare Hospital, Shenzhen 518172, China | | | Wanyu Xie | Department of Gynecology and Obstetrics, The First Affiliated Hospital of University of South China, Hengyang 421001, China | |
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| Abstract: |
| Objective: The aim of the study was to investigate the effect of SGI-1776 combination with DDP in sub-toxic concentration on induction of apoptosis of human ovarian cancer HO-8910 cells in vitro and to unravel the associated mechanisms. Methods: Human ovarian cancer HO-8910 cells were cultured in vitro. The inhibitory effect of SGI-1776 combination with DDP in sub-toxic concentration on induction on viability of human ovarian cancer HO-8910 cells was evaluated by the MTT assay. Cell apoptosis rate was analyzed by flow cytometry. The proteins expression level related to apoptosis were analyzed by Western blot. Results: SGI-1776 combination with DDP in sub-toxic concentration significantly inhibited the proliferation of human ovarian cancer HO-8910 cells, and proliferation inhibition rate was increased drastically compared with normal saline (NS) group or DDP group in sub-toxic concentration or SGI-1776 group in sub-toxic concentration (P﹤0.01). Apoptosis rate markedly increased after the treatment of SGI-1776 combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of Bax and Cyto-c were depressed by SGI-1776 combination with DDP in sub-toxic concentration. Conclusion: SGI-1776 combination with DDP in sub-toxic concentration could inhibit the cell proliferation and lead to cell apoptosis inhuman ovarian cancer HO-8910 cells, and its mechanism may be related to through mitochondrial apoptotic pathway. |
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