| Xiaoyan Gao,Xingcong Ma,Yinan Ma,Xinghuan Xue,Shuqun Zhang. The effect of baicalein on the expression of SATB1 in MDA-MB-231 cells. Oncol Transl Med, 2014, 13: 503-508. |
| 黄芩素对人乳腺癌MDA-MB-231细胞株SATB1表达的影响 |
| The effect of baicalein on the expression of SATB1 in MDA-MB-231 cells |
| Received:September 29, 2014 Revised:October 26, 2014 |
| DOI：10.1007/s10330-314-0012-x |
| 中文关键词: 黄芩素; SATB1; 乳腺癌; 增殖; 迁移 |
| 英文关键词: baicalein; special AT-rich sequence binding protein 1 (SATB1); breast cancer; proliferation; migration |
| 基金项目:国家自然科学基金面上项目（编号：81274136）、西安交通大学交叉项目基金（Xjj2012141）和西安交通大学第二附属医院人才基金（RCCGG201105）资助 |
| Author Name | Affiliation | E-mail | | Xiaoyan Gao* | The Second Affiliated Hospital of Xi’an Jiaotong University | gaoxiaoyan986@126.com | | Xingcong Ma | The Second Affiliated Hospital of Xi’an Jiaotong University | cameo1190@163.com | | Yinan Ma | The Second Affiliated Hospital of Xi’an Jiaotong University | yinanxue@126.com | | Xinghuan Xue | The Second Affiliated Hospital of Xi’an Jiaotong University | xxhjdey@126.com | | Shuqun Zhang | The Second Affiliated Hospital of Xi’an Jiaotong University | zhangshuqun1971@aliyun.com |
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| 中文摘要: |
| 目的：观察黄芩素对人乳腺癌MDA-MB-231细胞SATB1蛋白表达的影响。方法：用MTT法、划痕愈合实验观察不同浓度黄芩素干预后MDA-MB-231细胞增殖和运动迁移能力的变化；用WesternBlot法检测黄芩素干预后MDA-MB-231细胞SATB1蛋白表达的变化。结果：随作用时间的延长和药物浓度的增加，黄芩素对MDA-MB-231细胞增殖和运动迁移的抑制作用逐渐增强，呈明显的时间-剂量依赖性（P<0.05）；黄芩素可明显降低MDA-MB-231细胞中SATB1蛋白的表达，而且随着药物浓度的提高SATB1蛋白表达量逐渐减少（P<0.05）。结论：黄芩素可通过抑制SATB1的表达抑制肿瘤细胞的恶性增殖、侵袭和迁移能力。 |
| 英文摘要: |
| Objective: Baicalein had been proved to have anti-cancer activity in vitro and in vivo, including the inhibition of malignant proliferation, migration, adhesion and invasion of many kinds of cancer cells. The special AT-rich sequence binding protein 1 (SATB1) is a tissue-specific expression of nuclear matrix-binding protein and is reported to be a breast cancer “gene group organizer”. Previous studies have shown that SATB1 is involved in the growth, metastasis and prognosis of breast cancer. The present study was aimed to investigate whether baicalein inhibits the proliferation and migration of MDA-MB-231 human breast cancer cells through down-regulation of the SATB1 expression. Methods: MDA-MB-231 cells were treated for 24 h, 48 h and 72 h with various concentrations of baicalein (0, 5, 10, 20, 40 and 80 μM) respectively. Then, the proliferation and migration of MDA-MB-231 cells following treatment with baicalein were determined using colorimetric 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) and wound healing assays. Thereafter, western blot analysis was performed to detect the changes of SATB1 protein expression in MDA-MB-231 cells. Results: Along with the prolongation of time and increase of drug concentration, inhibitory effect of baicalein on proliferation and migration of MDA-MB-231 cells gradually increased, in a time- and dose- dependent manner (P < 0.05). Meanwhile, after treated with baicalein in different concentrations for 48 h, the level of SATB1 protein expression of MDA-MB-231 cells decreased obviously, in a dose-dependent manner (P < 0.05). Conclusion: Baicalein inhibits breast cancer cell proliferation and suppresses its invasion and metastasis by reducing cell migration possibly by down-regulation of the SATB1 protein expression, indicating that baicalein is a potential therapeutic agent for human breast cancer. |
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