| Yonghong Zhang,Yongxiang Li. A traditional Chinese medicine Huaier triggers G1 cell cycle arrest and apoptosis through cyclins-CDKs-CKIs machinery in MOLT4 cells. Oncol Transl Med, 2014, 13: 603-608. |
| 中药槐耳可能通过Cyclin-CDKs-CKIs途径引起MOLT4细胞G1期阻滞和诱导凋亡 |
| A traditional Chinese medicine Huaier triggers G1 cell cycle arrest and apoptosis through cyclins-CDKs-CKIs machinery in MOLT4 cells |
| Received:September 26, 2014 Revised:December 13, 2014 |
| DOI：10.1007/s10330-314-0010-z |
| 中文关键词: 中药；凋亡；细胞周期阻滞；MOLT4 |
| 英文关键词: Chinese medicine; apoptosis; cell cycle arrest; MOLT4 |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Yonghong Zhang | yong-hong zhang: first author,medical doctor,chief physician,E-mail:@qqcom Yong-xiang Li: corresponding author | 379847410@qq.com | | Yongxiang Li* | Anhui Medical University affiliated First Hospital | 379847410@qq.com |
|
| Hits: 11394 |
| Download times: 12313 |
| 中文摘要: |
| 目的 研究中药槐耳对体外MOLT4细胞系的细胞周期调控方面的影响。方法 使用MTT比色法检测MOLT4细胞活力，使用流式细胞术检测细胞周期和凋亡，使用Western blot检测细胞周期相关蛋白和凋亡相关蛋白的表达。 结果 槐耳能通过G1期阻滞和诱导凋亡使MOLT4细胞活性降低。槐耳作用于MOLT4细胞引起剂量和时间依赖性凋亡。槐耳阻滞MOLT4细胞在G1期的原因是Cdki蛋白(p19INK4,p21Cip/waf1 和 p27Kip1)表达增高，同时Cdk2, Cdk4, Cdk6, cyclin D1和 cyclin E表达降低。槐耳也引起凋亡相关蛋白Bax，Bcl-2和Caspase-3的表达。结论 首次证明了槐耳通过G1期阻滞和诱导凋亡抑制MOLT4细胞的增殖，这表明槐耳是一个细胞周期特异性的抗癌药物。 |
| 英文摘要: |
| Objective: The purpose of the study was to study the effect of Huaier, a traditional Chinese medicine, on the
cell cycle adjustment in MOLT4 cells in vitro. Methods: We used MTT assay to test cell viability, flow cytometry to detect cell
cycle and apoptosis and western blot to examine the expression of cell-cycle and apoptotic proteins in MOLT4 cells induced by Huaier. Results: Huaier could reduce the viability of MOLT4 cell by inducing G1 arrest and apoptosis. The induction of apoptosis after treatment with Huaier for 24 h was demonstrated in a dose- and time-dependent manner by flow cytometry analysis. G1 arrest induced by Huaier was modulated through the increased expression of Cdki proteins (p21cip/waf1 and p27kip1) with a simultaneous decrease in Cdk2, Cdk4, Cdk6, cyclin D1 and cyclin E expression. Huaier also induced Bax and Bcl-2 expression and activation of Caspase-3. Conclusion: It is firstly demonstrated that Huaier can inhibit proliferation of MOLT4 cells via G1 arrest and apoptosis. These results suggest that Huaier is a cell-cycle anti-cancer drug. |
|
View Full Text
Download reader HTML全文 |
| Close |