| Yuezhi Zhang,Hanchen Liu,Mengwen Li,Lijun Kong. The Effect and significance on Akt and PTEN Expression in melanoma B16 Cells with chamaejasme extract. Oncol Transl Med, 2014, 13: 600-602. |
| 黑蒜提取液对恶性黑色素瘤B16细胞中Akt和PTEN表达的影响及意义 |
| The Effect and significance on Akt and PTEN Expression in melanoma B16 Cells with chamaejasme extract |
| Received:September 22, 2014 Revised:October 17, 2014 |
| DOI：10.1007/s10330-314-0008-6 |
| 中文关键词: 恶性黑色素瘤；狼毒提取液；Akt；PTEN；免疫细胞化学 |
| 英文关键词: malignant melanoma; chamaejasme extract; Akt chamaejasme; PTEN; immunocytochemistry |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Yuezhi Zhang | Biochemistry and molecular biology laboratory, Binzhou Medical College, Yantai, Shandong 264003, China | jingxuanlemontre@tom.com | | Hanchen Liu | Department of radiotherapy , The PLA 107 hospital, Yantai, Shandong 264002, China | 49463515@qq.com | | Mengwen Li | Department of radiotherapy , The PLA 107 hospital, Yantai, Shandong 264002, China | yousun_mo@163.com | | Lijun Kong* | Biochemistry and molecular biology laboratory, Binzhou Medical College, Yantai, Shandong 264003, China | kong_lijun@163.com |
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| 中文摘要: |
| 目的 探讨狼毒提取液对恶性黑色素瘤B16细胞中Akt和PTEN表达的影响及其对恶性黑色素瘤增殖及凋亡的作用。方法 用免疫细胞化学法对经不同浓度狼毒提取液处理后的B16细胞中Akt、PTEN进行检测，原位标记法记录细胞凋亡指数（AI）。结果 经不同浓度狼毒提取液处理后的B16细胞中Akt的表达明显低于空白对照组，而PTEN的表达则显著上调，且呈剂量相关性（P<0.05）；不同浓度狼毒提取液处理后的B16细胞Akt / AI明显低于空白对照组，组间均数差异有统计学意义（F=24.58，P<0.05）。结论 狼毒提取液可通过下调Akt和上调PTEN的表达而抑制恶性黑色素瘤B16细胞的增殖并诱导其凋亡。 |
| 英文摘要: |
| Objective: The aim of this study is to investigate the effect on Akt and PTEN expression and the mechanism of proliferation and apoptosis of melanoma B16 cells treated with chamaejasme extract. Methods: The expressions of Akt and PTEN of B16 cells treated with different concentrations of chamaejasme extract were detected with immunohistochemical method, cell apoptosis index (AI) was calculated with in situ labeling method. Results: Akt expressions of B16 cells treated with different concentrations of chamaejasme extract were significantly lower than the control group, while the PTEN expressions significantly upregulated, and the effects appeared to be dose-related(P<0.05). The Akt/AI of B16 cells treated with different concentrations of chamaejasme extract was significantly lower than the control group, all the parameters had extremely difference(F=24.58,P<0.05. Conclusion: Chamaejasme extract could inhibit proliferation and induce apoptosis of malignant melanoma B16 cells by down-regulating the expression of Akt and up-regulating the expression of PTEN. |
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