| Dengfu Yao. Diagnostic and monitoring Values of Circulating AFP mRNA and HS-GGT for Hepatocellular Carcinoma. Oncol Transl Med, 2014, 13: 457-463. |
| 循环血AFP mRNA与HS-GGT检测对肝癌的诊断与监测价值 |
| Diagnostic and monitoring Values of Circulating AFP mRNA and HS-GGT for Hepatocellular Carcinoma |
| Received:August 21, 2014 Revised:October 06, 2014 |
| DOI：10.1007/s10330-014-0049-5 |
| 中文关键词: 肝癌；AFP mRNA；肝癌特异性GGT；诊断；转移 |
| 英文关键词: Hepatocellular carcinoma; AFP mRNA; Hepatoma-specific gamma- glutamyl transferase; Diagnosis; Metastasis |
| 基金项目:Supported by the Grants from the National Natural Science Foundation (81200634), the Projects of Jiangsu Medical Science (WSW-011, HK201102, BL2012053) |
| Author Name | Affiliation | E-mail | | Dengfu Yao* | Research Center of Clinical Medicine, Affiliated Hospital of Nantong University
| yaodf@ahnmc.com |
|
| Hits: 10969 |
| Download times: 12213 |
| 中文摘要: |
| 目的: 肝癌(HCC)早期发现和转移监测极为重要，本文分析了外周血肝癌特异性GGT (HS-GGT)及单核细胞(PBMCs)中AFP-mRNA在肝癌诊断及转移监测中的临床价值. 方法: 从肝癌组织或外周血PBMC制备总RNA, 经逆转录合成AFP-cDNA, 以巢式PCR扩增和测序证实并定量循环血中HS-GGT活性，比较评估两标志物的临床价值. 结果: AFP-mRNA扩增片段与原设定完全一致(159 bp) 并经测序证实，总RNA最低检出灵敏度为2 ng/L. 肝癌组AFP-mRNA和HS-GGT阳性率分别为60.4 % (113 of 187)和84.5 % (158 of 187),显著地高于其它各组(P < 0.01). 在AFP < 50 ng/mL肝癌病人中，HS-GGT和AFP-mRNA阳性率分别为78.2 %和56.4 %. 肝部患者伴肝外转移者AFP-mRNA全数阳性（100%）.两标志在肿瘤大小组间未见明显差异，两者联合诊断其总阳性率为93.6 %.结论: 血中HS-GGT异常或出现AFP-mRNA均为肝癌诊断与鉴别的特异性标志，尤其前者可早期诊断肝癌，后者可用于肝癌转移监测. |
| 英文摘要: |
| BACKGROUD The early finding of hepatocellular carcinoma (HCC) and monitoring of its metastasis are of the utmost importance. The objectives of the study were to investigate the values of circulating hepatoma-specific γ-glutamyl transferase (HS-GGT) fraction and α-fetoprotein mRNA (AFP-mRNA) from peripheral blood mononuclear cells (PBMCs) in diagnosis or monitoring metastasis of HCC.
METHODS Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients.
RESULTS The amplified fragments of AFP-mRNA were identical to original designed ones (159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP- mRNA and HS-GGT were 60.4 % (113 of 187) and 84.5 % (158 of 187) in the HCC group, and their significantly higher (P < 0.01) than that in any of other groups. The high frequencies of HS-GGT and AFP-mRNA were 78.2 % and 56.4 % in the HCC patients with AFP less than 50 ng/mL. All positive of AFP-mRNA was found in HCC patients with extrahepatic metastasis. No significant correlation was found between tumor size and AFP-mRNA or HS-GGT. The total incidence of combined AFP-mRNA and HS-GGT detection was 93.6 % for HCC diagnosis.
CONCLUSION The abnormal HS-GGT and presence of AFP-mRNA are specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC. |
|
View Full Text
Download reader HTML全文 |
| Close |