| Tenglong Zhang,Weili Jia,Li Sun,Xiaoyan Wang,Xiuhua Xing,Shuai Gong,Chunrui Yu. Expression and significance of microRNA-32 in multiple myeloma. Oncol Transl Med, 2014, 13: 472-475. |
| microRNA-32在多发性骨髓瘤中的表达及临床意义 |
| Expression and significance of microRNA-32 in multiple myeloma |
| Received:August 18, 2014 Revised:September 29, 2014 |
| DOI：10.1007/s10330-014-0047-7 |
| 中文关键词: miR-32；多发性骨髓瘤；实时荧光定量PcR |
| 英文关键词: microRNA-32 (miR-32); multiple myeloma (MM); real-time polymerase chain reaction (real-time PCR) |
| 基金项目:青岛市2012年度医药科研指导计划资助项目(2012-wSZD042)；青岛市公共领域科技支撑计划资助项目(20loKZJ-9) |
| Author Name | Affiliation | E-mail | | Tenglong Zhang | Department of Oncology, Qingdao Municipal Hospital | 331376656@qq.com | | Weili Jia | Department of Oncology, Qingdao Municipal Hospital | | | Li Sun | Department of Oncology, Qingdao Municipal Hospital | | | Xiaoyan Wang | Department of Oncology, Qingdao Municipal Hospital | | | Xiuhua Xing | Department of Oncology, Qingdao Municipal Hospital | | | Shuai Gong | Department of Oncology, Qingdao Municipal Hospital | | | Chunrui Yu* | Department of Oncology, Qingdao Municipal Hospital | yuyouyoudangdang@sina.com |
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| 中文摘要: |
| 目的探讨micmRNA-32(miR-32)在多发性骨髓瘤(MM)骨髓单个核细胞(BMMNc)中的表达水平及意义。方法收集43例MM患者及20例正常对照的骨髓标本，采用实时荧光定量PcR法检测BMMNc中miR-32的表达，并分析其与Durie-salmon (D-s)分期和p：-微球蛋白(p：-MG)水平的关系。结果miR-32在MM中的表达水平(5.29±0.31)明显高于正常对照组(相对值为1)，复发／难治组miR-32表达水平(6.86±0.24)明显高于初治组(4.15±0.29)，差异均有统计学意义(P<0.05)。MM患者化疗后miR-32表达水平较化疗前明显降低(5.29±0.31vs3.43±0.45)，化疗有效组降低十分显著(4.57±0.41vs1.92±0.15)，差异均有统计学意义(P<0.05)；无效／进展组miR一32表达水平化疗前后无明显改变。miR-32表达与MM D-S分期和B：-MG水平有关。结论miR-32表达在MM的发生、发展中发挥着重要作用，有望成为MM疾病进展和疗效的预测指标。 |
| 英文摘要: |
| Objective: This study was aimed to explore the expression of microRNA-32 (miR-32) in multiple myeloma (MM), and study its association with β2-microglobulin and staging of MM by Durie-Salmon classification. Methods: The expression level of miR-32 in bone marrow mono-nuclear cells of MM were examined by real-time polymerase chain reaction (real-time PCR), and the correlations between the expression level of miR-32 and related clinic pathologic features β2-microglobulin, staging of MM by Durie-Salmon classification were further analyzed. Results: The expression of miR-32 in MM patients was obviously higher than that in normal control (P < 0.05). The expression of miR-32 in relapsed/refractory MM patients was obviously higher than that in newly diagnosed MM patients. The expression of miR-32 in MM patients decreased after chemical therapy than that before treatment, especially in effective group (P < 0.05). There was no statistically significant change in ineffective/progress group after chemical therapy (P > 0.05) .The expression of miR-32 was associated to staging of MM and β2-microglobulin level. Conclusion: Expression level of miR-32 in MM patients is significantly higher than that in normal bone marrow, these data indicated that miR-32 may play an important role in the development of MM. High-regulated expression of miR-32 was associated with β2-microglobulin and staging of MM by Durie-Salmon classification. |
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