| Lilin Hang,Min Zhang,Fanliang Meng,Mei Zhong,Jing Li. ZNF217 expression correlates with the biological behavior of human ovarian cancer cells. Oncol Transl Med, 2014, 13: 539-544. |
| 锌指蛋白217基因对人卵巢浆液性囊腺癌上皮细胞HO-8910生物学行为的影响 |
| ZNF217 expression correlates with the biological behavior of human ovarian cancer cells |
| Received:August 05, 2014 Revised:October 23, 2014 |
| DOI：10.1007/s10330-014-0044-x |
| 中文关键词: 锌指蛋白217；卵巢肿瘤；肿瘤转移；转染 |
| 英文关键词: ovarian cancer; zinc-finger protein 217 (ZNF217) gene; gene expression; proliferation; invasion; tumor metastasis |
| 基金项目:国家自然科学基金(30973193)；广东省医学科学技术研究基金(wsTJJ201111104401041974JD5153780) |
| Author Name | Affiliation | E-mail | | Lilin Hang | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515 | 352795915@qq.com | | Min Zhang | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Fanliang Meng | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Mei Zhong | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Jing Li* | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | lijing7405@126.com |
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| 中文摘要: |
| 目的：探讨锌指蛋白217(zNF217)基因对人卵巢浆液性囊腺癌上皮细胞HO-8910生物学行为的影响。方法：将pEGFP-N1-zNF217和pEGFP-N1质粒分别转染入H0-89l0细胞，采用逆转录聚合酶链反应(RT—PCR)和western blot法检测转染后pEGFP-N1-ZNF17／H0-8910、pEGFP-N1／HO-8910和H0_8910细胞中zNF217 mRNA和蛋白的表达；采用四甲基偶氮唑蓝(MTT)法检测3组细胞的体外增殖能力；采用Boyden小室侵袭实验检测3组细胞的体外侵袭能力。将pEGFP-Nl-zNF217/HO-8910和pEGFP-N1/HO-8910细胞接种于裸鼠皮下，建立裸鼠移植瘤模型，观察pEGFP-N1-zNF217/H0_8910和pEGFP-N1/H0-8910细胞的体内成瘤能力。结果RT—PCR和western blot法检测结果均显示，转染后的pEGFP-N1-zNF217/H0—89lO细胞中zNF217基因的表达增加。MTT法检测结果显示，pEGFP-N1-zNF217/H0-89lo细胞的体外增殖能力明显强于pEGFP-N1/HO-8910细胞和H0-8910细胞(P<0．001)。Boyden小室侵袭实验的结果显示，pEGFP-N1-zNF 217/HO-8910、pEGFP-N1/HO-8910和HO-8910细胞的穿膜细胞数分别为(141.25±13.91)个/200倍视野、(82.50±11.73)个/200倍视野和(81.75±12.12)个/200倍视野，差异统计学意义(F=29.247，P<0.001)。裸鼠成瘤实验的结果显示，pEGFP-N1-zNF217／HO-8910细胞的体内成瘤能力明显强于pEGFP-N1/H0-8910细胞(P<0.001)。结论zNF217基因具有促进卵巢浆液性囊腺癌上皮细胞增殖和侵袭转移的能力，zNF217基因的表达可作为卵巢癌侵袭转移过程中一个有价值的指标。 |
| 英文摘要: |
| Objective: The aim of the study was to investigate the correlation of zinc-finger protein 217 (ZNF217) gene expression with the biological behavior of human ovarian cancer HO-8910 cells. Methods: The expression of ZNF217 in ovarian carcinoma cell lines was detected by RT-PCR and Western blot, respectively. The biological behaviors of the transfectants were investigated by MTT, in vitro Boyden chamber and in vivo invasion assay, respectively. Results: RT-PCR and Western blotting revealed that transfection of ZNF217 into the HO-8910 cells significantly increased their proliferation along with markedly enhanced in vitro and in vivo invasion and metastatic abilities. MTT assay showed that the proliferation ability of pEGFPN1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells and HO-8910 cells (P < 0.001). The Boyden chamber assay showed that the numbers of migrating pEGFP-N1-ZNF217/HO-8910, pEGFP-N1/ HO-8910 and HO-8910 cells were (141.25 ± 13.91) cells /200 × field, (82.50 ± 11.73) cells /200 × field and (81.75 ± 12.12) cells /200 × field, respectively, with a significant difference between them (F = 29.274, P < 0.001). The nude mouse experiment showed that the in vivo tumor formation ability of pEGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells (P < 0.001). Conclusion: Based on these clinical and laboratory observations, we conclude that ZNF217 may contribute to ovarian cancer invasion and metastasis, and associated with worse clinical outcomes. We evaluated ZNF217’s role as a biomarker of ovarian carcinogenesis and tumor progression in patient samples and explored possible molecular mechanisms in promoting tumor growth and invasion. |
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