| Yuxuan Che,Xiuhua Sun,Chaomei Huang,Jinbo Zhao. Influence of Tamoxifen or the combination of Tamoxifen and Cisplatin on the growth of human lung adenocarcinoma A549 cells. Oncol Transl Med, 2014, 13: 523-527. |
| 他莫昔芬单药及联合DDP对人肺腺癌A549细胞株生长影响的实验研究 |
| Influence of Tamoxifen or the combination of Tamoxifen and Cisplatin on the growth of human lung adenocarcinoma A549 cells |
| Received:July 15, 2014 Revised:October 21, 2014 |
| DOI：10.1007/s10330-014-0034-z |
| 中文关键词: 他莫昔芬 DDP 化疗增敏 A549细胞株 |
| 英文关键词: Tamoxifen; Cisplatin (DDP); chemosensitization; A549 cells |
| 基金项目:2009年辽宁省科技厅 代码：2009225009-5 |
| Author Name | Affiliation | E-mail | | Yuxuan Che | The Second Affiliated Hospital of Dalian Medical University | cheyuxu_an_ok@126.com | | Xiuhua Sun | The Second Affiliated Hospital of Dalian Medical University | | | Chaomei Huang | The Second Affiliated Hospital of Dalian Medical University | | | Jinbo Zhao* | The Second Affiliated Hospital of Dalian Medical University | zhaojinbo66@sina.com |
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| 中文摘要: |
| 目的：1.探讨他莫昔芬（Tamoxifen，TAM）对人肺腺癌A549细胞株增殖与凋亡的影响。2.探讨他莫昔芬联合DDP对人肺腺癌A549细胞株生长影响，为肺癌的临床治疗提供新的方向。
方法：本实验采用人肺腺癌A549细胞株作为研究对象，不同浓度的他莫昔芬作用于A549细胞株72小时，用流式细胞术（FCM）检测他莫昔芬对人肺腺癌A549细胞株细胞周期及细胞凋亡的影响。以DDP单独给药及与无细胞毒作用浓度的他莫昔芬联合给药的方式，分别作用于人肺腺癌A549细胞株，作用时间为72小时，用MTT法检测DDP单独给药及与他莫昔芬联合给药对人肺腺癌A549细胞株的细胞毒性作用，测定其吸光度(absorbance，A)值，计算抑制率。所有数据均以均数加减标准差（ ±s）表示，统计学处理采用统计软件包SPSS13.0处理。采用单因素方差分析不同浓度他莫昔芬各组间差异，采用X2检验分析联合用药各组间差异，P<0.05为差异有统计学意义。
结果：他莫昔芬作用人肺腺癌A549细胞72小时后，S期与G2/M期比例下降，G0/G1期的细胞运动阻滞。从总的趋势上看，静止期细胞增多，增殖细胞减少，使人肺腺癌细胞增殖受影响。他莫昔芬对人肺腺癌A549细胞具有诱导凋亡作用，其凋亡率随药物浓度的升高而上升，具有明显的剂量依赖关系。无细胞毒作用浓度的他莫昔芬(1umol/L)与DDP联用后的抑制率较单用DDP明显提高，并且随药物浓度增高而增高(P<0.01)，DDP的半数抑制浓度(IC50)由11.2ug/ml降至2.9 ug/ml。
结论：1.他莫昔芬对人肺腺癌A549细胞具有抑制增殖作用及诱导凋亡作用。2.无细胞毒作用浓度的他莫昔芬与DDP联合对人肺腺癌A549细胞具有化疗增敏作用。 |
| 英文摘要: |
| Objective: The experiment aims to investigate the influence of Tamoxifen and the combination of Tamoxifen and Cisplatin (DDP) on the growth of human lung adenocarcinoma A549 cells. Methods: We treated human lung adenocarcinoma A549 cells with different concentrations of Tamoxifen, DDP and combination of DDP and Tamoxifen with non-toxicity for 72 h. Then we calculated the inhibition rate through MTT approach and detected the apoptosis rate by flow cytometry. The statistical analysis was performed with SPSS 13.0 software and statistical differences were determined by one-way ANOVA. The data were expressed as the mean ± standard deviation and all experiments were performed in three times. The value of P < 0.05 was considered to indicate a statistically significant difference. Results: 1. The inhibition rates of Tamoxifen with 2.5 μmol/L, 5 μmol/L, 10 μmol/L and 20 μmol/L on the growth of the A549 cells were 18.7%, 25.8%, 54% and 98.8%, respectively (P = 0.000). Tamoxifen with concentration of 1 μmol/L has no obvious cytoxicity on the A549 cells (P > 0.05). 2. As the increase concentration of Tamoxifen, the S stage and G2/M of the A549 cells decreased while the G0/G1 increased. The apoptosis rate of Tamoxifen with 0 μmol/L, 0.1 μmol/L, 1 μmol/L and 10 μmol/L on the A549 cells were 6.51%, 8.91%, 17.97% and 42.7%, respectively. 3. The inhibition rates of combination of Tamoxifen with 1 μmol/L and DDP with 1.25 μg/mL, 2.5 μg/mL, 5 μg/mL, 10 μg/mL and 20 μg/mL on the A549 cells were 40.4%, 54.4%, 72.9%, 86.1% and 92.4%, respectively (P < 0.05). Conclusion: Tamoxifen can inhibit the proliferation of human lung adenocarcinoma A549 cells and induce the apoptosis of the A549 cells. The combination of Tamoxifen with non-toxicity and DDP can improve the sensitivity of chemotherapy on the A549 cells. |
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