| Yuxuan Che,Meixiang Zhou,Peng Zhan,Tiantian Zou,Xiuhua Sun. The anti-cancer effect of Huaier aqueous extract with rh-Endostatin and DDP. Oncol Transl Med, 2014, 13: 349-354. |
| 槐耳颗粒单药及联合rh-Endostatin、DDP抗肿瘤作用的研究 |
| The anti-cancer effect of Huaier aqueous extract with rh-Endostatin and DDP |
| Received:July 01, 2014 Revised:July 29, 2014 |
| DOI：10.1007/s10330-014-0026-z |
| 中文关键词: 槐耳颗粒；rh-Endostatin；抗肿瘤作用；人肺腺癌A549细胞；人肺腺癌A549/DDP细胞 |
| 英文关键词: Huaier aqueous extract; rh-Endostatin; anti-cancer effects; A549 cells; A549/DDP cells |
| 基金项目:辽宁省自然科学基金 |
| Author Name | Affiliation | E-mail | | Yuxuan Che | The Second Affiliated Hospital of Dalian Medical University | cheyuxu_an_ok@126.com | | Meixiang Zhou | The Second Affiliated Hospital of Dalian Medical University | | | Peng Zhan | The Second Affiliated Hospital of Dalian Medical University | | | Tiantian Zou | The Second Affiliated Hospital of Dalian Medical University | | | Xiuhua Sun* | The Second Affiliated Hospital of Dalian Medical University | 3038668@vip.sina.com |
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| 中文摘要: |
| 目的：探讨槐耳颗粒及其与重组人血管内皮抑制素(rh-Endostatin)、顺铂（DDP）联合用药时对人肺腺癌细胞株A549的抑制作用及诱导凋亡作用。也探讨槐耳颗粒逆转人肺腺癌细胞株A549/DDP的耐药作用。方法：将处于对数生长期的人肺腺癌A549细胞用不同浓度的槐耳颗粒及槐耳颗粒联合rh-Endostatin、DDP的培养液培养24h、36h、48h，运用四氮唑蓝（MTT）比色法检测吸光度（OD）值，计算抑制率；用流式细胞仪检测细胞凋亡率。将人肺腺癌A549细胞和A549/DDP细胞分别用不同浓度的DDP、槐耳颗粒及rh-Endostatin的培养液培养72h。结果：1、槐耳颗粒对肺腺癌细胞系A549有明显的抑制作用，其抑制率随浓度的升高而上升。2、rh-Endostatin联合浓度为4mg/ml的槐耳颗粒在24h、36h、48h及rh-Endostatin联合浓度为2mg/ml的槐耳颗粒在48h时对人肺腺癌A549细胞的抑制率均有增加(p<0.005)。3、DDP联合浓度为2mg/ml或4mg/ml的槐耳颗粒对人肺腺癌A549细胞的抑制率有所增加(p<0.005)。4、槐耳颗粒联合DDP及槐耳颗粒联合rh-Endostatin、DDP对人肺腺癌A549细胞的抑制率均有增加(p<0.005)。结论：1、槐耳颗粒对人肺腺癌A549细胞具有抑制作用及诱导凋亡作用。槐尔颗粒与rh-Endostatin或DDP联合对A549细胞的抑制具有相加作用。槐尔颗粒、rh-Endostatin、DDP联合对A549细胞的抑制具有协同作用。2、槐耳颗粒能够逆转人肺腺癌A549/DDP细胞的耐药性。 |
| 英文摘要: |
| Objective: The aim of our study was to explore the inhibition and apoptosis-inducing effect of the combination of Huaier aqueous extract with recombinant human Endostatin and DDP in human lung adenocarcinoma A549 cells. We also investigated the reversal effect of Huaier aqueous extract in reversing cisplatin resistance in human lung adenocarcinoma A549/DDP cells. Methods: We treated A549 cells with Huaier aqueous extract and the combination of Huaier aqueous extract and DDP or rh-Endostatin for 24 h, 36 h and 48 h. And then we calculated the inhibition rate through MTT approach and detected the apoptosis rate by flow cytometry. We also treated A549 and A549/DDP cells with DDP, Huaier aqueous extract, DDP and Huaier aqueous extract for 72 h, respectively. Results: Huaier aqueous extract can inhibit the growth of A549 cells and the inhibition rate improved with the increase of the concentration. The inhibition rate of the combination of rh-Endostatin and 4 mg/mL of Huaier aqueous extract in three time points and the combination of rh-Endostatin and 2 mg/mL of Huaier aqueous extract in the time point of 48 h on the growth of A549 cells all improved (P < 0.005). The inhibition rate of the combination of DDP and Huaier aqueous extract with the concentration of 2 mg/mL or 4 mg/mL on the growth of A549 cells all improved (P < 0.005). The combination of Huaier aqueous extract and DDP and the combination of Huaier aqueous extract with rh-Endostatin and DDP can improve the inhibition on the growth of A549 cells (P < 0.005). Conclusion: Huaier aqueous extract has the inhibition and apoptosis-inducing effects on the A549 cells. And the combination of Huaier aqueous extract and rh-Endostatin or DDP has the synergistic effects on the inhibition of A549 cells. The combination of Huaier aqueous extract with rh-Endostatin and DDP has the synergistic effects on the inhibition of A549 cells. Huaier aqueous extract can reverse the cisplatin resistance in human lung adenocarcinoma A549/DDP cells. |
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