| Zhihua Wang,li Qin,Chunyan Zhang,Yuyan Ma. Effect of IL-12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC-823. Oncol Transl Med, 2014, 13: 355-359. |
| Effect of IL-12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC-823 |
| Received:July 01, 2014 Revised:July 28, 2014 |
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| KeyWord:Cytokine-induced killer (CIK) cell; interleukin-12 (IL-12); proliferative ability; cytotoxicity |
| Author Name | Affiliation | E-mail | | Zhihua Wang | Hefei Fenghuang Tumor Hospital, Hefei 267800, China
Cancer Research Institute of Harbin Medical University, Harbin 150081, China | hljwzh000@163.com | | li Qin | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | | | Chunyan Zhang | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | | | Yuyan Ma | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | |
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| Abstract: |
| Objective: The aim of the study was to evaluate the effect of interleukin-12 (IL-12) on the proliferation and cytotoxicity of cytokine-induced killer (CIK) cells in vitro. Methods: Three different combinations of cytokines, IL-2, IL-12 IL-2, and IL-12, were used to proliferate CIK cells, adding IFN-γ, IL-1 and CD3 McAb in each one. Phenotype of the cells was analyzed by flow cytometry. The cellular proliferation and cytotoxic activity were determined by cytometry and MTT assay. Results: CIK cells generated by the three methods showed high reproductive activity, and no obviously difference in inducing CD3 CD56 cells was found among the three groups. The group of IL-2 IL-12 evidently enhanced both the proliferation and the cytotoxicity of the CIK cells compared with the other two groups (P < 0.05). Conclusion: IL-12 could be used to induce the CIK cells as well as IL-2. CIK cells induced by combining IL-12 with IL-2 had stronger proliferative ability and higher cytotoxicity
to tumor cells in vitro, which could be used as a potential anti-tumor adoptive immunotherapy in clinic. |
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