| Zhihua Wang,li Qin,Chunyan Zhang,Yuyan Ma. Effect of IL-12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC-823. Oncol Transl Med, 2014, 13: 355-359. |
| IL-12对胃腺癌细胞BGC-823诱导的杀伤细胞的增值和细胞毒性作用 |
| Effect of IL-12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC-823 |
| Received:July 01, 2014 Revised:July 28, 2014 |
| DOI：10.1007/s10330-014-0021-4 |
| 中文关键词: 细胞因子诱导的杀伤细胞；白介素-12；增殖能力；细胞毒性 |
| 英文关键词: Cytokine-induced killer (CIK) cell; interleukin-12 (IL-12); proliferative ability; cytotoxicity |
| 基金项目:本课题受国家“九五”重点攻关课题（96-960A-01-20）和黑龙江省重点攻关课题（G00C190401）基金资助 |
| Author Name | Affiliation | E-mail | | Zhihua Wang* | Hefei Fenghuang Tumor Hospital, Hefei 267800, China
Cancer Research Institute of Harbin Medical University, Harbin 150081, China | hljwzh000@163.com | | li Qin | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | | | Chunyan Zhang | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | | | Yuyan Ma | Cancer Research Institute of Harbin Medical University, Harbin 150081, China | |
|
| Hits: 8567 |
| Download times: 10381 |
| 中文摘要: |
| 目的：观察IL-12对细胞因子诱导的杀伤细胞（cytokine-induced killer cells, CIK）体外扩增的影响。方法：采用三种不同的细胞因子组合扩增CIK细胞，即IL-2组：IFN-γ、CD3单抗、IL-1和IL-2； IL-2加IL-12组：IFN-γ、CD3单抗、IL-1、IL-2和IL-12；IL-12组：IFN-γ、CD3单抗、IL-1和IL-12。细胞计数法测定细胞的增殖、流式细胞术分析细胞表型，MTT法测定细胞毒作用。 结果：三种方法均可使细胞增殖能力显著增加，对CD3 CD56 细胞的诱导作用也无明显差异，IL-12与IL-2联合作用组促增殖能力和细胞毒性作用明显强于其他两组（P<0.05）。结论：IL-12同样可以用于CIK细胞的诱导，而联合IL-12与IL-2可以显著增强CIK细胞的增殖能力和细胞毒性。 |
| 英文摘要: |
| Objective: The aim of the study was to evaluate the effect of interleukin-12 (IL-12) on the proliferation and cytotoxicity of cytokine-induced killer (CIK) cells in vitro. Methods: Three different combinations of cytokines, IL-2, IL-12 IL-2, and IL-12, were used to proliferate CIK cells, adding IFN-γ, IL-1 and CD3 McAb in each one. Phenotype of the cells was analyzed by flow cytometry. The cellular proliferation and cytotoxic activity were determined by cytometry and MTT assay. Results: CIK cells generated by the three methods showed high reproductive activity, and no obviously difference in inducing CD3 CD56 cells was found among the three groups. The group of IL-2 IL-12 evidently enhanced both the proliferation and the cytotoxicity of the CIK cells compared with the other two groups (P < 0.05). Conclusion: IL-12 could be used to induce the CIK cells as well as IL-2. CIK cells induced by combining IL-12 with IL-2 had stronger proliferative ability and higher cytotoxicity
to tumor cells in vitro, which could be used as a potential anti-tumor adoptive immunotherapy in clinic. |
|
View Full Text
Download reader HTML全文 |
| Close |