Min Tang,Jun Bai,Chunyan Chen,Yingxia Ning,Xiaochun Li,Hanzhen He. The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro. Oncol Transl Med, 2014, 13: 427-431. |
The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro |
Received:June 30, 2014 Revised:August 29, 2014 |
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KeyWord:ovarian cancer; Laggera alata flavonen (LAF); apoptosis |
Author Name | Affiliation | E-mail | Min Tang | Hunan Provice Mawangdui Hospital, Changsha 410016, China | shushuanlao@163.com | Jun Bai | Hangzhou Red Cross Hospital, Hangzhou 310003, China | | Chunyan Chen | Hunan Provice Mawangdui Hospital, Changsha 410016, China | | Yingxia Ning | The First Affiliated Hospital of Guangzhou Medical University, Guangzhou | shushuanlao@163.com | Xiaochun Li | Department of Obstetrics and Gynecology, Longgang District Maternal and Child Healthcare Hospital of Shenzhen, | | Hanzhen He | Department of Information, Longgang District Maternal and Child Healthcare Hospital of Shenzhen, Shenzhen | |
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Abstract: |
Objective: The purpose of this study was to investigate the effect of Laggera alata flavonen (LAF) on the inhibiting effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods: Human ovarian
cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide (PI) staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results: LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group (P < 0.05), and the IC50 was 4.28 μg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable difference compared with NS group (P < 0.05). Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion: LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro. |
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