| Min Tang,Jun Bai,Chunyan Chen,Yingxia Ning,Xiaochun Li,Hanzhen He. The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro. Oncol Transl Med, 2014, 13: 427-431. |
| 六棱菊总黄酮体外抑制人卵巢癌HO-8910细胞增殖及其机制 |
| The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro |
| Received:June 30, 2014 Revised:August 29, 2014 |
| DOI：10.1007/s10330-014-0015-2 |
| 中文关键词: 卵巢癌；六棱菊总黄酮；凋亡 |
| 英文关键词: ovarian cancer; Laggera alata flavonen (LAF); apoptosis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Min Tang | Hunan Provice Mawangdui Hospital, Changsha 410016, China | shushuanlao@163.com | | Jun Bai | Hangzhou Red Cross Hospital, Hangzhou 310003, China | | | Chunyan Chen | Hunan Provice Mawangdui Hospital, Changsha 410016, China | | | Yingxia Ning* | The First Affiliated Hospital of Guangzhou Medical University, Guangzhou | shushuanlao@163.com | | Xiaochun Li | Department of Obstetrics and Gynecology, Longgang District Maternal and Child Healthcare Hospital of Shenzhen, | | | Hanzhen He | Department of Information, Longgang District Maternal and Child Healthcare Hospital of Shenzhen, Shenzhen | |
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| 中文摘要: |
| 目的 研究六棱菊总黄酮(LAF)体外抑制人卵巢癌HO-8910细胞增殖的作用及其可能的分子生物学机制。方法 体外培养人卵巢癌HO-8910细胞，用MTT检测LAF对HO-8910细胞的增殖抑制作用，用AO/EB 染色和FCM检测LAF对HO-8910细胞的凋亡诱导作用，用western blot检测HO-8910细胞Fas、Caspase-8、tBid、Cyto-c蛋白表达情况。结果LAF对HO-8910细胞的增殖抑制呈浓度依赖性，与NS组比较，组间差异有统计学意义(P<0.05)，LAF作用48h的IC50值为4.28 μg/mL；AO/EB 染色显示LAF作用HO-8910细胞48h后，细胞出现典型的凋亡形态学改变，凋亡率呈浓度依赖性，与NS组比较，组间差异有统计学意义(P<0.05)，伴随Fas、Caspase-8、tBid、Cyto-c蛋白表达上调，与NS组比较差异有统计学意义(P<0.05)。结论LAF通过上调Fas、Caspase-8、Bid、Cyto-c蛋白表达，诱导HO-8910细胞凋亡，抑制增殖。 |
| 英文摘要: |
| Objective: The purpose of this study was to investigate the effect of Laggera alata flavonen (LAF) on the inhibiting effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods: Human ovarian
cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide (PI) staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results: LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group (P < 0.05), and the IC50 was 4.28 μg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable difference compared with NS group (P < 0.05). Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion: LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro. |
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